CAJ | 학술논문

目的：研究外源绿色荧光蛋白（ green fluorescent protein，简称GFP）基因在BALB/c绿色荧光裸鼠主要器官组织中的表达及其差异。方法小动物成像系统和RT-PCR方法检测GFP的组织分布以及荧光表达水平情况。结果经活体荧光影像系统观察及PCR方法检测发现GFP可以在裸鼠多个器官组织中表达，其中在胰腺、心脏、全脑、皮肤、睾丸中表达量较高。结论外源绿色荧光蛋白可以在模型动物体内成功表达且稳定遗传，其中在胰腺组织中高表达。
목적：연구외원록색형광단백（ green fluorescent protein，간칭GFP）기인재BALB/c록색형광라서주요기관조직중적표체급기차이。방법소동물성상계통화RT-PCR방법검측GFP적조직분포이급형광표체수평정황。결과경활체형광영상계통관찰급PCR방법검측발현GFP가이재라서다개기관조직중표체，기중재이선、심장、전뇌、피부、고환중표체량교고。결론외원록색형광단백가이재모형동물체내성공표체차은정유전，기중재이선조직중고표체。
Objective To study whether the green fluorescent protein ( GFP) gene can be successfully expressed in green fluorescent nude mice and the tissue distribution characteristics.Methods Small animal imaging system and RT-PCR assay were used to detect the GFP tissue distribution and fluorescence expression level.Results The GFP can be expressed in multiple tissues in green fluorescent nude mice.A higher expression was observed in the pancreas, heart, brain, and skin.Conclusion Exogenous GFP can be stably expressed and inherited in green fluorescent nude mice, with the highest expression in the pancreas.