中国实验动物学报
中國實驗動物學報
중국실험동물학보
ACTA LABORATORIUM ANIMALIS SCIENTIA SINICA
2014年
5期
53-58
,共6页
去卵巢大鼠%全身振动%跑台运动%金雀异黄酮%子宫
去卵巢大鼠%全身振動%跑檯運動%金雀異黃酮%子宮
거란소대서%전신진동%포태운동%금작이황동%자궁
Ovariectomized rats%Whole-body vertical vibration%Treadmill exercise%Genistein%Uterus
目的：观察全身垂直振动、跑台运动和金雀异黄酮对去卵巢骨质疏松大鼠子宫重量指数和组织形态学以及糖原合成激酶3β（ GSK-3β）蛋白表达的影响。方法将72只3月龄雌性SD大鼠按体重分层后随机分为假手术组（12只）和去卵巢组（60只）。去卵巢10周时，将去卵巢组大鼠按体重分层后又随机分为去卵巢组、振动组、跑台组、金雀异黄酮组和雌激素组（每组8～10只），并开始进行不同干预处理。干预处理8周时，于末次处理结束36～48h内，腹主动脉取血处死各组大鼠，用电子天平称量大鼠子宫的重量，用HE染色方法观察子宫形态学的变化，用Western blot方法检测子宫GSK-3β和P-GSK-3β蛋白表达的变化。结果与去卵巢组比较，雌激素组大鼠子宫重量指数显著增加，而振动组、跑台组、金雀异黄酮组大鼠子宫重量指数均无显著变化；与去卵巢组比较，雌激素组、跑台组和振动组大鼠子宫P-GSK-3β/GSK-3β蛋白的比值均显著增加，而金雀异黄酮组无显著变化。结论全身垂直振动和跑台运动均能刺激去卵巢骨质疏松大鼠子宫GSK-3β蛋白的磷酸化，而金雀异黄酮无此效应。
目的：觀察全身垂直振動、跑檯運動和金雀異黃酮對去卵巢骨質疏鬆大鼠子宮重量指數和組織形態學以及糖原閤成激酶3β（ GSK-3β）蛋白錶達的影響。方法將72隻3月齡雌性SD大鼠按體重分層後隨機分為假手術組（12隻）和去卵巢組（60隻）。去卵巢10週時，將去卵巢組大鼠按體重分層後又隨機分為去卵巢組、振動組、跑檯組、金雀異黃酮組和雌激素組（每組8～10隻），併開始進行不同榦預處理。榦預處理8週時，于末次處理結束36～48h內，腹主動脈取血處死各組大鼠，用電子天平稱量大鼠子宮的重量，用HE染色方法觀察子宮形態學的變化，用Western blot方法檢測子宮GSK-3β和P-GSK-3β蛋白錶達的變化。結果與去卵巢組比較，雌激素組大鼠子宮重量指數顯著增加，而振動組、跑檯組、金雀異黃酮組大鼠子宮重量指數均無顯著變化；與去卵巢組比較，雌激素組、跑檯組和振動組大鼠子宮P-GSK-3β/GSK-3β蛋白的比值均顯著增加，而金雀異黃酮組無顯著變化。結論全身垂直振動和跑檯運動均能刺激去卵巢骨質疏鬆大鼠子宮GSK-3β蛋白的燐痠化，而金雀異黃酮無此效應。
목적：관찰전신수직진동、포태운동화금작이황동대거란소골질소송대서자궁중량지수화조직형태학이급당원합성격매3β（ GSK-3β）단백표체적영향。방법장72지3월령자성SD대서안체중분층후수궤분위가수술조（12지）화거란소조（60지）。거란소10주시，장거란소조대서안체중분층후우수궤분위거란소조、진동조、포태조、금작이황동조화자격소조（매조8～10지），병개시진행불동간예처리。간예처리8주시，우말차처리결속36～48h내，복주동맥취혈처사각조대서，용전자천평칭량대서자궁적중량，용HE염색방법관찰자궁형태학적변화，용Western blot방법검측자궁GSK-3β화P-GSK-3β단백표체적변화。결과여거란소조비교，자격소조대서자궁중량지수현저증가，이진동조、포태조、금작이황동조대서자궁중량지수균무현저변화；여거란소조비교，자격소조、포태조화진동조대서자궁P-GSK-3β/GSK-3β단백적비치균현저증가，이금작이황동조무현저변화。결론전신수직진동화포태운동균능자격거란소골질소송대서자궁GSK-3β단백적린산화，이금작이황동무차효응。
Objective The aim of this study was to observe the effects of whole-body vertical vibration, treadmill exercise, genistein administration and estrogen injection on the uterus weight index and uterus histology as well as the ex-pression of glycogen synthase kinase ( GSK-3β) protein in the uterus of ovariectomized osteoporotic rats.Methods Sev-enty-two healthy 3-month old female SD rats were randomly divided into two groups by body weight:sham-operation group (Sham) and ovariectomized group (OVX).At 10 weeks after OVX operation, the OVX rats were randomly divided into the following five groups by body weight: OVX group, whole-body vertical vibration group (WBVV), treadmill exercise group ( TX) , genistein group ( G) and 17β-estrogen group ( E2 ) .Then they were treated with different methods according to the experiment design.At the end of experiment, the uterus weight was measured with an electronic balance.The uterus histology was observed with HE staining and the expressions of GSK-3βand P-GSK-3βproteins were detected by Western blot.Results Apart from E2 treatment, all the other three treatments did not increase the uterus weight and the thickness of endometrium compared with that in the OVX rats.Apart from genistein treatment, all the other three treatments increased the ratio of protein expression of P-GSK-3βto GSK-3βcompared with that in the OVX rats.Conclusions Both whole-body vertical vibration and treadmill exercise can stimulate the phosphorylation of GSK-3βin the uterus of OVX osteoporotic rats.However, genistein has no such stimulation effect.
