CAJ | 학술논문

目的：寻找能调节T细胞功能的相关分子，进行与T细胞介导的自身免疫性疾病相关的研究。方法从BALB/c小鼠骨髓中收集树突状细胞，免疫Wistar大鼠，进行细胞融合，建立杂交瘤细胞系。筛选得到很多株能调节T细胞功能的杂交瘤细胞系，对其中一株最能抑制T细胞增殖的杂交瘤细胞系进行了进一步的深入研究。结果显示其目标分子是CD45，同时增殖实验结果显示该抗体能显著抑制T细胞增殖反应。结论抗CD45单克隆抗体能有效抑制T细胞增殖，有望将本抗体用于T细胞介导的自身免疫性疾病的相关预防及治疗中。
목적：심조능조절T세포공능적상관분자，진행여T세포개도적자신면역성질병상관적연구。방법종BALB/c소서골수중수집수돌상세포，면역Wistar대서，진행세포융합，건립잡교류세포계。사선득도흔다주능조절T세포공능적잡교류세포계，대기중일주최능억제T세포증식적잡교류세포계진행료진일보적심입연구。결과현시기목표분자시CD45，동시증식실험결과현시해항체능현저억제T세포증식반응。결론항CD45단극륭항체능유효억제T세포증식，유망장본항체용우T세포개도적자신면역성질병적상관예방급치료중。
Objective To identify molecules that modulate T-cell functions and serve the studies on T-cell media-ted autoimmune diseases.Methods Bone marrow-derived dentritic cells were collected from BALB/c mice to immunize Wistar rats, and to establish many hybridoma cell lines.Many hybridoma cell lines which could modulate T-cell functions were obtained.One of the cell lines, most actively inhibiting T-cell proliferation, was further studied.Results The anti-CD45 mAb recognized CD45 and significantly suppressed T-cell proliferation in proliferation assays.Conclusions Our re-sults indicate that the anti-CD45 mAb can effectively suppress T-cell proliferation, and is promising to be used in the pre-vention and treatment of T-cell mediated autoimmune diseases in the future.