CAJ | 학술논문

目的：通过比较脂多糖（ LPS）和石墨粉颗粒分别诱导小鼠急性肺损伤的病理形态学差异，探讨不同来源细颗粒物成分导致急性肺损伤的可能机制。方法将140只SPF级18～20 g雄性KM小鼠随机分为7组，除正常对照组外其他组经气管内分别滴注LPS溶液及石墨粉混悬液制备急性肺损伤小鼠。记录各组动物死亡率，光镜和透射电镜下观察各组小鼠不同时间点肺组织病理变化。 Western Blot检测肺组织中NE的蛋白表达，实时定量PCR法检测肺组织中MCP-1的mRNA表达。结果与正常对照组相比，G （石墨粉）组和L （ LPS）组均有不同程度病理学改变，G组小鼠肺部有大量巨噬细胞渗出，L组小鼠肺部渗出物以中性粒细胞为主；肺组织中NE蛋白表达均高于正常对照组（P＜0.05），且L组与G组之间差异有显著性（P＜0.05）；肺组织中MCP-1mRNA表达均高于正常对照组（P＜0.01），且L组与G组之间也有显著差异（P＜0.01）。结论不同来源颗粒物引起肺部的病理损伤不同，可能引起炎症反应的机制也存在差异，即成分复杂的细颗粒物导致急性肺损伤的机制可能存在混合性。
목적：통과비교지다당（ LPS）화석묵분과립분별유도소서급성폐손상적병리형태학차이，탐토불동래원세과립물성분도치급성폐손상적가능궤제。방법장140지SPF급18～20 g웅성KM소서수궤분위7조，제정상대조조외기타조경기관내분별적주LPS용액급석묵분혼현액제비급성폐손상소서。기록각조동물사망솔，광경화투사전경하관찰각조소서불동시간점폐조직병리변화。 Western Blot검측폐조직중NE적단백표체，실시정량PCR법검측폐조직중MCP-1적mRNA표체。결과여정상대조조상비，G （석묵분）조화L （ LPS）조균유불동정도병이학개변，G조소서폐부유대량거서세포삼출，L조소서폐부삼출물이중성립세포위주；폐조직중NE단백표체균고우정상대조조（P＜0.05），차L조여G조지간차이유현저성（P＜0.05）；폐조직중MCP-1mRNA표체균고우정상대조조（P＜0.01），차L조여G조지간야유현저차이（P＜0.01）。결론불동래원과립물인기폐부적병리손상불동，가능인기염증반응적궤제야존재차이，즉성분복잡적세과립물도치급성폐손상적궤제가능존재혼합성。
Objective To compare the differences of lung pathological changes of acute lung injury in mice in-duced by lipopolysaccharide ( LPS) and graphite particles, and to explore the possible mechanisms of acute lung injury in-duced by fine particles of different origins.Methods 140 male specific-pathogen-free Kunming mice weighing 18-20 g were randomly divided into 7 experimental groups, in addition to the normal control group.The experimental groups were treated by intratracheal instillation of LPS solution or graphite powder suspension in different doses, respectively, to induce acute lung injury in the mice.The mortality of the mice was observed, and pathological changes of the lung tissues were ex-amined by light and transmission electron microscopy.Western blot was used to detect the protein expression of neutrophil elastase ( NE) in lung tissues , and real-time quantitative PCR was used to detect mRNA expression of monocyte chemotac-tic protein-1 ( MCP-1) in the lung tissue .Results Compared with the normal control group, some pathological changes were observed in the lung tissues of the groups L ( LPS) and G ( graphite) .There were numerous macrophages in the lung tissues in the group G mice, and exudate, mainly neutrophils, in the lung tissues of the group L.The NE protein expres-sion in the lung tissue was significantly higher than that of the normal control group ( P<0.05) , and there was also a sig-nificant difference between the groups L and G (P<0.05).The MCP-1 mRNA expression in lung tissues was higher in the control group (P<0.01), and there was also a significant difference between the groups L and G (P<0.01).Conclu-sions Diverse types of particulate matters induce different pathological changes in the lungs, therefore the mechanism may also be different in the inflammatory responses.It means that the lung injuries caused by fine particles of mixed composition may have complex mechanisms.