CAJ | 학술논문

利用 TALE-TFs在小鼠成纤维细胞中激活β-酪蛋白基因启动子表达载体
이용 TALE-TFs재소서성섬유세포중격활β-락단백기인계동자표체재체
Direct activation of expression vector withβ-casein gene promoter using engineered TALE transcription factors in mouse fibroblasts

万方数据

中国实验动物学报中國實驗動物學報 중국실험동물학보
ACTA LABORATORIUM ANIMALIS SCIENTIA SINICA
2014年 5期 13-16 ,共4页

皮文辉%梁龙%唐红%张译元%郭延华%王立民%向春和%周平%刘守仁

피문휘%량룡%당홍%장역원%곽연화%왕립민%향춘화%주평%류수인

TALE-TFs%小鼠成纤维细胞%β-酪蛋白基因启动子%表达调控 TALE-TFs%小鼠成纖維細胞%β-酪蛋白基因啟動子%錶達調控
TALE-TFs%소서성섬유세포%β-락단백기인계동자%표체조공
Transcription activator-like effector-transcription factors (TALE-TFs)%Mouse fibroblasts%β-casein gene promoter%Gene expression modulation

目的：利用TALE-TFs，在小鼠成纤维细胞中激活β-酪蛋白基因启动子，为检测β-酪蛋白基因启动子-目的基因表达框的表达结果，提供一种检测途径。方法将构建的TALE-TFs和β-酪蛋白基因启动子-Red报告基因质粒电转染进入小鼠成纤维细胞，通过荧光显微镜直接观察报告基因表达情况。结果与结论利用TALE人工转录因子，在小鼠成纤维细胞中能够激活β-酪蛋白基因启动子表达框，为替代乳腺上皮细胞表达验证系统提供了新的途径。
목적：이용TALE-TFs，재소서성섬유세포중격활β-락단백기인계동자，위검측β-락단백기인계동자-목적기인표체광적표체결과，제공일충검측도경。방법장구건적TALE-TFs화β-락단백기인계동자-Red보고기인질립전전염진입소서성섬유세포，통과형광현미경직접관찰보고기인표체정황。결과여결론이용TALE인공전록인자，재소서성섬유세포중능구격활β-락단백기인계동자표체광，위체대유선상피세포표체험증계통제공료신적도경。
Objective TALE-TFs were adopted to provide a new way in detection of the expression result ofβ-ca-sein gene promoter-interesting gene expression cassettes in mouse fibroblasts.Methods TALE-TFs of eukaryotic expres-sion plasmid and expression cassette withβ-casein gene promoter and red fluorescent protein reporter gene were co-nucleo-fected into mouse fibroblasts by Amaxa nucleofector.Results and Conclusion β-casein gene promoter was activated by artificial TALE-TFs in the mouse fibroblasts.The way is a new expression verification system instead of mammary epithelial cells with fibroblasts.