CAJ | 학술논문

通过构建萝卜抽薹开花相关基因的 cDNA 文库，获取大量的 EST 序列，并与十字花科模式植物拟南芥、白菜等进行比较基因组学研究，研究结果显示：萝卜与拟南芥、白菜等植物基因组具有很高的同源性，控制抽薹开花的相关基因在不同物种中具有保守性。拟南芥抽薹研究表明，抽薹开花过程受多基因控制，具有多种调控途径。抽薹开花是一个复杂的过程，其中 FLC 、FT 、LFY 等基因处于关键位置。目前这些关键基因在萝卜 EST序列中已经发现了同源片段，并进行了一些基因克隆，结合萝卜基因组测序的完成，未来萝卜抽薹研究将进入基因功能验证和全基因组调控模式研究之中。
통과구건라복추대개화상관기인적 cDNA 문고，획취대량적 EST 서렬，병여십자화과모식식물의남개、백채등진행비교기인조학연구，연구결과현시：라복여의남개、백채등식물기인조구유흔고적동원성，공제추대개화적상관기인재불동물충중구유보수성。의남개추대연구표명，추대개화과정수다기인공제，구유다충조공도경。추대개화시일개복잡적과정，기중 FLC 、FT 、LFY 등기인처우관건위치。목전저사관건기인재라복 EST서렬중이경발현료동원편단，병진행료일사기인극륭，결합라복기인조측서적완성，미래라복추대연구장진입기인공능험증화전기인조조공모식연구지중。
Based on a radish cDNA library,a lot of EST sequences are obtained and a compara-tive genomic research is carried out between cruciferous model plants such as Arabidopsis thali-ana and cabbage.The results show that radish is in high homology with Arabidopsis thaliana and cabbage.Related genes are conserved about bolting and flowering traits in different species. Studies in bolting traits of Arabidopsis show that the process of bolting and flowering is con-trolled by many genes,and there are a variety of regulatory pathways.It is a complex process, including FLC 、FT 、LFY genes in a critical position.Currently the homologous fragments of these key genes have been found and cloned in radish EST sequences,combined with the com-pletion of the genomic sequencing of radish.Future researches will explore the function of genes and genome-wide regulation model.