CAJ | 학술논문

利用ISSR分子标记技术对云南省选育出的49个板栗优良品种进行遗传多样性和亲缘关系分析。结果表明，筛选出的11条引物一共扩增出86条DNA谱带，其中70条为多态性条带，多态位点百分率（ PPB ％）81.40％，多态性指数（H）0.228， Shannon信息指数（I）0.350，数据表明所选育的板栗优良品种资源具有较高的遗传多样性。根据各品种的Nei’ s遗传距离，进行UPGMA聚类，49个品种被分为2个大类，其中第1大类又分为了2个亚类，来源地相同的品种基本聚在同一类。聚类结果在分子水平上揭示了各板栗品种的亲缘关系，为今后板栗育种的亲本选配提供科学依据。
이용ISSR분자표기기술대운남성선육출적49개판률우량품충진행유전다양성화친연관계분석。결과표명，사선출적11조인물일공확증출86조DNA보대，기중70조위다태성조대，다태위점백분솔（ PPB ％）81.40％，다태성지수（H）0.228， Shannon신식지수（I）0.350，수거표명소선육적판률우량품충자원구유교고적유전다양성。근거각품충적Nei’ s유전거리，진행UPGMA취류，49개품충피분위2개대류，기중제1대류우분위료2개아류，래원지상동적품충기본취재동일류。취류결과재분자수평상게시료각판률품충적친연관계，위금후판률육충적친본선배제공과학의거。
Inter-simple Sequence Repeat ( ISSR) molecular markers were used to analyze genetic diversity and re-lations of 49 chestnut cultivars (Castanea mollissima).86 bands were amplified by 11 selected primers, among which 70 bands were polymorphic .Percentage of polymorphic loci was 81.4%, the mean expected heterozygosity (H) was 0.228 and the Shannon information index (I) was 0.350.The results indicated that selected chestnut cultivars had a high level of genetic diversity .According to Nei ’ s genetic distances , 49 cultivars were clustered into 2 groups and 4 subgroups by the UPGMA analysis . The clustering accorded mainly with their introduced places.The genetic relationship of all 49 chestnut cultivars revealed by the ISSR dendrogram would be help to ac-curately select the parents in chestnut breeding programs .