CAJ | 학술논문

根据肉葡萄球菌(Staphylococcus carnosus)TM300的基因组序列设计引物，经 PCR 扩增得到其 tufA 基因的启动子Peftu片段与大肠杆菌-葡萄球菌穿梭载体pBT2-Tat-GFP连接，构建了穿梭质粒pBT2-ETG．结果表明，穿梭质粒 pBT2-ETG 成功地转入大肠杆菌与肉葡萄球菌宿主中稳定表达具有活性的绿色荧光蛋白 GFP，并被转运到肉葡萄球菌宿主的细胞壁，为进一步研究肉葡萄球菌双精氨酸(Tat)转运系统正确分泌其他外源蛋白奠定了实验基础．
근거육포도구균(Staphylococcus carnosus)TM300적기인조서렬설계인물，경 PCR 확증득도기 tufA 기인적계동자Peftu편단여대장간균-포도구균천사재체pBT2-Tat-GFP련접，구건료천사질립pBT2-ETG．결과표명，천사질립 pBT2-ETG 성공지전입대장간균여육포도구균숙주중은정표체구유활성적록색형광단백 GFP，병피전운도육포도구균숙주적세포벽，위진일보연구육포도구균쌍정안산(Tat)전운계통정학분비기타외원단백전정료실험기출．
According to the genome sequence of Staphylococcus carnosus TM300 genome,a pair of primers was designed for PCR amplification of promoter Peftu of tufA gene. The PCR-amplified promoter Peftu fragment was cloned into plasmid pBT2-Tat-GFP and chemically transformed into E. coli host. A shuttle vector pBT2-ETG was thereby constructed and succ-essfully transformed into E. coli and S. carnosus TM300 hosts,respectively. The experimental results reveal that GFP was expressed by the E. coli-Staphylococcus shuttle vector pBT2-ETG in both E. coli and S. carnosus hosts. The expressed GFP was translocated to the cell walls of S. carnosus host in fluorescent active form. Thereby,a preliminary experimental basis was laid for the further study of other exogenous protein secretion via twin-arginine translocation pathway in S. carnosus.