CAJ | 학술논문

目的：研究基质金属蛋白酶（ MMP）-7、MMP-26在滋养细胞疾病中的表达及与研究滋养细胞肿瘤相关因素之间的关系，为早期临床诊断滋养细胞疾病，判断其恶性程度提供有价值的线索。方法应用免疫组织化学S-ABC 法检测10例正常绒毛，15例完全性葡萄胎，5例部分性葡萄胎，14例侵蚀性葡萄胎，8例绒癌组织中MMP-7、MMP-26的表达，进行统计学分析。结果①MMP-7在正常绒毛组、葡萄胎组、侵蚀性葡萄胎组、绒癌组表达的阳性率分别为60．000％、45．000％、85．714％、100．000％，且有明显的统计学差异（χ2＝11．011，P＝0．012），同时，MMP-7阳性率与胎盘绒毛滋养细胞非正常增生有显著相关性（r＝7．709， P＝0．005），正常绒毛组、葡萄胎组分别和侵蚀性葡萄胎组、绒癌组之间阳性率比较具有明显的统计学差异（P＜0．05）；②MMP-26在正常绒毛组、葡萄胎组、侵蚀性葡萄胎组、绒癌组的表达阳性率分别为90．000％、45．000％、35．714％、25．000％，且有明显的差异（χ2＝9．680，P＝0．021），同时，MMP-26阳性率与胎盘绒毛滋养细胞非正常增生有显著相关性（r＝7．519，P＝0．006）。正常绒毛组、葡萄胎组、侵蚀性葡萄胎组、绒癌组MMP-26阳性率两两之间比较均有明显差异（P＜0．05）。结论 MMP-7高表达MMP-26低表达可能提示妊娠滋养细胞侵袭性增强，对葡萄胎清宫组织检测MMP-7、MMP-26表达强度，有助于判断葡萄胎的侵袭性强弱。
목적：연구기질금속단백매（ MMP）-7、MMP-26재자양세포질병중적표체급여연구자양세포종류상관인소지간적관계，위조기림상진단자양세포질병，판단기악성정도제공유개치적선색。방법응용면역조직화학S-ABC 법검측10례정상융모，15례완전성포도태，5례부분성포도태，14례침식성포도태，8례융암조직중MMP-7、MMP-26적표체，진행통계학분석。결과①MMP-7재정상융모조、포도태조、침식성포도태조、융암조표체적양성솔분별위60．000％、45．000％、85．714％、100．000％，차유명현적통계학차이（χ2＝11．011，P＝0．012），동시，MMP-7양성솔여태반융모자양세포비정상증생유현저상관성（r＝7．709， P＝0．005），정상융모조、포도태조분별화침식성포도태조、융암조지간양성솔비교구유명현적통계학차이（P＜0．05）；②MMP-26재정상융모조、포도태조、침식성포도태조、융암조적표체양성솔분별위90．000％、45．000％、35．714％、25．000％，차유명현적차이（χ2＝9．680，P＝0．021），동시，MMP-26양성솔여태반융모자양세포비정상증생유현저상관성（r＝7．519，P＝0．006）。정상융모조、포도태조、침식성포도태조、융암조MMP-26양성솔량량지간비교균유명현차이（P＜0．05）。결론 MMP-7고표체MMP-26저표체가능제시임신자양세포침습성증강，대포도태청궁조직검측MMP-7、MMP-26표체강도，유조우판단포도태적침습성강약。
Objective To study the expressions of MMP-7 and MMP-26 in trophoblastic disease and their relationship with trophoblastic tumor related factors, so as to provide valuable clues for early clinical diagnosis of trophoblastic disease and determination of malignant degree.Methods The protein expressions of MMP-7 and MMP-26 in 10 cases of normal villi, 15 cases of complete hydatidiform mole, 5 cases of partial hydatiform mole, 14 cases of invasive mole and 8 cases of choriocarcinama were detected using immunohistochemistry assay ( S-ABC) for statistic analysis.Results The positive rate of MMP-7 expression in normal villi group, hydatidiform mole group, invasive mole group and choriocarcinama group was 60.000%, 45.000%, 85.714% and 100.000%, respectively, and there was significant difference (χ2 =11.011, P=0.012).Meanwhile, MMP-7 positive rate was significantly correlated with the abnormal proliferation of placental trophoblast cell (r=7.709, P=0.005).The differences between normal villi group, mole group and invasive mole group and choriocarcinoma group were significant (P <0.05).The positive rate of MMP-26 expression in normal villi group, hydatidiform mole group, invasive mole group and choriocarcinama group was 90.000%, 45.000%, 35.714%and 25.000%, respectively, and there was statistically significant difference (χ2 =9.680,P =0.021).Meanwhile, MMP-26 positive rate was significantly correlated with the abnormal proliferation of placental trophoblast cell (r=7.519, P=0.006).Group comparison of normal villi group, hydatidiform mole group, invasive mole group and choriocarcinama group was significantly different (P<0.05).Conclusion High expression of MMP-7 and low expression of MMP-26 may indicate enhanced gestational trophoblastic invasion.Detection of MMP-7 and MMP-26 expression in hydatidiform mole tissues is helpful to identify the invasive degree of hydatidiform mole.