CAJ | 학술논문

中药五陪子能够有效预防和治疗龋齿，但其抗龋活性成分和机制尚待研究．本实验应用不同剂量五倍子大极性非酚性成分处理根面龋大鼠模型，Bowen记分法评价实验动物根面龋情况， ELISA法检测大鼠龈沟液内基质金属蛋白酶-9（MMP-9）的表达变化．结果发现1 mg/mL及2 mg/mL五倍子处理组第一磨牙根面龋记分及龈沟液内MMP-9含量均显著低于蒸馏水处理组和0.5 mg/mL五倍子处理组（P＜0.01），与氟化钠处理组间无显著性差异（P＞0.05），且两组间比较无显著性差异（P＞0.05）．说明五倍子大极性非酚性物质能够通过抑制龋齿饮食大鼠龈沟液内MMP-9表达以抑制根面龋进展，最佳浓度为1 mg/mL．
중약오배자능구유효예방화치료우치，단기항우활성성분화궤제상대연구．본실험응용불동제량오배자대겁성비분성성분처리근면우대서모형，Bowen기분법평개실험동물근면우정황， ELISA법검측대서간구액내기질금속단백매-9（MMP-9）적표체변화．결과발현1 mg/mL급2 mg/mL오배자처리조제일마아근면우기분급간구액내MMP-9함량균현저저우증류수처리조화0.5 mg/mL오배자처리조（P＜0.01），여불화납처리조간무현저성차이（P＞0.05），차량조간비교무현저성차이（P＞0.05）．설명오배자대겁성비분성물질능구통과억제우치음식대서간구액내MMP-9표체이억제근면우진전，최가농도위1 mg/mL．
Galla Chinensis plays an effective role in preventing and treating caries.However,the active compo-nent and its mechanism still remain unknown.Different doses of non-phenolic constituents of Galla Chinensis extracts(GCE)are used to treat root surface caries in SD rats.Root surface caries is estimated by a modified Bowen′s method,and the levels of matrix metalloproteinase-9(MMP-9)in gingival crevicular fluid are detec-ted by ELISA.The results indicate that Bowen′s scores and the levels of MMP-9 in moderate or high dose GCE group are much less than those in control group or low dose GCE group(P<0.01).These above indexes are sim-ilar to those in NaF group(P>0.05 ).There is no difference between moderate and high dose GCE groups(P>0.05 ).It is therefore concluded that the non-phenolic constituents of GCE can prevent the progress of root sur-face caries by inhibiting the expression of MMP-9 in rats.The optimum working concentration is 1 mg/mL.