药学与临床研究
藥學與臨床研究
약학여림상연구
PHARMACEUTICAL AND CLINICAL RESEARCH
2014年
5期
422-423
,共2页
亚油酸%小茴香%HPLC-ELSD%含量测定
亞油痠%小茴香%HPLC-ELSD%含量測定
아유산%소회향%HPLC-ELSD%함량측정
Linoleic acid%Foeniculum vulgare Mill.%HPLC-ELSD%Content determination
目的：建立小茴香中亚油酸含量的 HPLC-ELSD 测定方法并测定不同产地小茴香的亚油酸含量。方法：采用 Cosmosil 5 C18-MS-Ⅱ柱，甲醇-水-冰醋酸（95∶5∶0.1%），流速1.0 mL· min-1，柱温35℃，漂移管温度40℃，雾化气 N2，载气流速3.0 L·min-1。结果与结论：本法测定小茴香中亚油酸含量简便可靠。测得4个产地小茴香中亚油酸含量最高为8.73 mg·g-1，最低为1.70 mg·g-1。不同产地小茴香中亚油酸含量差异明显。
目的：建立小茴香中亞油痠含量的 HPLC-ELSD 測定方法併測定不同產地小茴香的亞油痠含量。方法：採用 Cosmosil 5 C18-MS-Ⅱ柱，甲醇-水-冰醋痠（95∶5∶0.1%），流速1.0 mL· min-1，柱溫35℃，漂移管溫度40℃，霧化氣 N2，載氣流速3.0 L·min-1。結果與結論：本法測定小茴香中亞油痠含量簡便可靠。測得4箇產地小茴香中亞油痠含量最高為8.73 mg·g-1，最低為1.70 mg·g-1。不同產地小茴香中亞油痠含量差異明顯。
목적：건립소회향중아유산함량적 HPLC-ELSD 측정방법병측정불동산지소회향적아유산함량。방법：채용 Cosmosil 5 C18-MS-Ⅱ주，갑순-수-빙작산（95∶5∶0.1%），류속1.0 mL· min-1，주온35℃，표이관온도40℃，무화기 N2，재기류속3.0 L·min-1。결과여결론：본법측정소회향중아유산함량간편가고。측득4개산지소회향중아유산함량최고위8.73 mg·g-1，최저위1.70 mg·g-1。불동산지소회향중아유산함량차이명현。
Objective: To establish a quantitative HPLC-ELSD method for the determination of linoleic acid in Foeniculum vulgare Mill. from different habitats. Methods: A column of Cosmosil 5C18-MS-Ⅱ was used with the mobile phase of methanol and water and acetic acid (95∶5∶0.1%), the fIow rate was 1.0 mL· min-1, and the column temperature was set at 35 ℃. The temperature of drift tube was set at 40 ℃ with an air fIow rate of 3.0 L·min-1 for ELSD. Results and Conclusion: The established HPLC-ELSD method is simple and suitable for the quality control of Foeniculum vulgare Mill. The maximum content of linoleic acid in Foeniculum vulgare Mill. from four habitats was 8.73 mg·g-1 and the minimum content was 1.70 mg· g-1. Great difference of linoleic acid content is shown in Foeniculum vulgare Mill. from different habitats.
