药学与临床研究
藥學與臨床研究
약학여림상연구
PHARMACEUTICAL AND CLINICAL RESEARCH
2014年
5期
409-411
,共3页
冯艳%乔进%陈惠%郑惠华
馮豔%喬進%陳惠%鄭惠華
풍염%교진%진혜%정혜화
灵芝多糖%糖尿病%氧化应激
靈芝多糖%糖尿病%氧化應激
령지다당%당뇨병%양화응격
Ganoderma lucidum polysaccharides%Diabetes mellitus%Oxidative stress
目的：研究灵芝多糖对链脲佐菌素（STZ）诱导的糖尿病大鼠胸主动脉内皮损伤的保护作用及对糖尿病大鼠胸主动脉氧化应激的影响。方法：SD 大鼠经过4周高脂饮食后腹腔注射链脲佐菌素（30 mg·kg-1）造模，随机分为正常对照组、模型组、灵芝多糖（GLPs）高、低剂量组。经过12周给药治疗后，测定血清中过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-PX）、总胆固醇（TC）和甘油三酯（TG）的水平；然后胸主动脉切片 HE 染色，观察胸主动脉超微结构及病理改变。结果：GLPs-H 组血糖浓度较模型组相比均明显下降（P<0.01），血清 CAT 和 GSH-PX 水平较模型组相比均明显上升（P<0.01）；血清 TC、TG 含量较模型组相比均明显下降（P<0.01）。 HE 染色，与模型组相比，GLPs-H 组光镜下主动脉内膜增厚、内皮损伤、中膜增厚的情况有所改善。结论：灵芝多糖可减轻糖尿病大鼠体内氧化应激水平，具有减轻脂质过氧化作用，对糖尿病胸主动脉并发症的发生发展有一定的预防作用。
目的：研究靈芝多糖對鏈脲佐菌素（STZ）誘導的糖尿病大鼠胸主動脈內皮損傷的保護作用及對糖尿病大鼠胸主動脈氧化應激的影響。方法：SD 大鼠經過4週高脂飲食後腹腔註射鏈脲佐菌素（30 mg·kg-1）造模，隨機分為正常對照組、模型組、靈芝多糖（GLPs）高、低劑量組。經過12週給藥治療後，測定血清中過氧化氫酶（CAT）、穀胱甘肽過氧化物酶（GSH-PX）、總膽固醇（TC）和甘油三酯（TG）的水平；然後胸主動脈切片 HE 染色，觀察胸主動脈超微結構及病理改變。結果：GLPs-H 組血糖濃度較模型組相比均明顯下降（P<0.01），血清 CAT 和 GSH-PX 水平較模型組相比均明顯上升（P<0.01）；血清 TC、TG 含量較模型組相比均明顯下降（P<0.01）。 HE 染色，與模型組相比，GLPs-H 組光鏡下主動脈內膜增厚、內皮損傷、中膜增厚的情況有所改善。結論：靈芝多糖可減輕糖尿病大鼠體內氧化應激水平，具有減輕脂質過氧化作用，對糖尿病胸主動脈併髮癥的髮生髮展有一定的預防作用。
목적：연구령지다당대련뇨좌균소（STZ）유도적당뇨병대서흉주동맥내피손상적보호작용급대당뇨병대서흉주동맥양화응격적영향。방법：SD 대서경과4주고지음식후복강주사련뇨좌균소（30 mg·kg-1）조모，수궤분위정상대조조、모형조、령지다당（GLPs）고、저제량조。경과12주급약치료후，측정혈청중과양화경매（CAT）、곡광감태과양화물매（GSH-PX）、총담고순（TC）화감유삼지（TG）적수평；연후흉주동맥절편 HE 염색，관찰흉주동맥초미결구급병리개변。결과：GLPs-H 조혈당농도교모형조상비균명현하강（P<0.01），혈청 CAT 화 GSH-PX 수평교모형조상비균명현상승（P<0.01）；혈청 TC、TG 함량교모형조상비균명현하강（P<0.01）。 HE 염색，여모형조상비，GLPs-H 조광경하주동맥내막증후、내피손상、중막증후적정황유소개선。결론：령지다당가감경당뇨병대서체내양화응격수평，구유감경지질과양화작용，대당뇨병흉주동맥병발증적발생발전유일정적예방작용。
Objective: To investigate the protective effects of ganoderma lucidum polysaccharide (GLPs) on the impairment of the aorta pectoralis in the STZ induced diabetes mellitus (DM) rats and to explore the effects of GLPs on the oxidative stress in DM rats. Methods: SD rats were fed with high fat diet for 4 weeks, then injected STZ (30 mg·kg-1) to replicate type 2 diabetic rats. The successful diabetic model rats were randomized into normal control group, diabetes group, Ganoderma lucidum polysaccharides of low and high-dose groups. After 12 week's treatment, the activity of catalase (CAT) and glutathione peroxidase (GSH-PX), the levels of total cholesterol (TC) and triglycerides (TG) in serum were detected. Slice of aorta pectoralis were stained with HE to observe ultramicro structure and patho-change of aorta pectoralis. Re-sults: The blood glucose concentration of the GLPs-H group was lower than that of the diabetes group (P<0.01); the activities of catalase and glutathione peroxidase in serum were significantly higher in the GLPs-H group than in the diabetes group (P<0.01); The levels of TC and TG in serum were significantly lower in the GLPs-H group than in the diabetes group (P<0.01). When observed under light microscope for HE stains, compared with the DM group with aortic tunica intima incrassation, injury of endodermis and tunica media incrassation, the GLPs-H group showed less damages. Conclusion: There is a confirmed action of GLPs in depressing oxidative stress and lipid peroxidation of the diabetes rats, and GLPs play a role in the prevention of diabetic complications of aorta pectoralis.
