华南师范大学学报（自然科学版）
華南師範大學學報（自然科學版）
화남사범대학학보（자연과학판）
JOURNAL OF SOUTH CHINA NORMAL UNIVERSITY （NATURAL SCIENCE EDITION）
2014年
5期
97-104
,共8页
郭晋雅%张称%王大创%高峰
郭晉雅%張稱%王大創%高峰
곽진아%장칭%왕대창%고봉
观赏%紫叶甘薯%遗传特性%ISSR%花色素%层次分析法
觀賞%紫葉甘藷%遺傳特性%ISSR%花色素%層次分析法
관상%자협감서%유전특성%ISSR%화색소%층차분석법
ornament%purple leaf sweet potato%inherited character%ISSR%anthocyanidin%analytic hierarchy process
以作者的实验室发现的变异单株‘紫叶甘薯’为材料，采用形态性状、花色素相对含量和ISSR为遗传标记，对其与普通甘薯品种（系）之间的遗传差异进行了分析，采用层次分析法，对其观赏开发价值进行了定量评价．结果表明：（1）‘紫叶甘薯’的地上器官均呈紫色，在20个形态标记中，‘紫叶甘薯’与9个甘薯品种（系）最明显的形态差异是茎叶的色彩；（2）‘紫叶甘薯’茎叶等地上器官的花色素含量明显高于其它品种（系），并且各器官之间的花色素含量存在明显差异，表现为叶＞叶柄＞茎；（3）使用9条引物从10个甘薯品种（系）中共扩增出93个DNA片段，其中多态性片段为78个，占总条带数的83.8％；依据ISSR分子标记绘制聚类图，‘紫叶甘薯’与‘白骨企龙’及‘渝紫263’的亲缘关系最近；（4）采用层次分析法对‘紫叶甘薯’的观赏开发价值进行定量评价的结果表明，‘紫叶甘薯’具有较高的观赏开发与利用价值．
以作者的實驗室髮現的變異單株‘紫葉甘藷’為材料，採用形態性狀、花色素相對含量和ISSR為遺傳標記，對其與普通甘藷品種（繫）之間的遺傳差異進行瞭分析，採用層次分析法，對其觀賞開髮價值進行瞭定量評價．結果錶明：（1）‘紫葉甘藷’的地上器官均呈紫色，在20箇形態標記中，‘紫葉甘藷’與9箇甘藷品種（繫）最明顯的形態差異是莖葉的色綵；（2）‘紫葉甘藷’莖葉等地上器官的花色素含量明顯高于其它品種（繫），併且各器官之間的花色素含量存在明顯差異，錶現為葉＞葉柄＞莖；（3）使用9條引物從10箇甘藷品種（繫）中共擴增齣93箇DNA片段，其中多態性片段為78箇，佔總條帶數的83.8％；依據ISSR分子標記繪製聚類圖，‘紫葉甘藷’與‘白骨企龍’及‘渝紫263’的親緣關繫最近；（4）採用層次分析法對‘紫葉甘藷’的觀賞開髮價值進行定量評價的結果錶明，‘紫葉甘藷’具有較高的觀賞開髮與利用價值．
이작자적실험실발현적변이단주‘자협감서’위재료，채용형태성상、화색소상대함량화ISSR위유전표기，대기여보통감서품충（계）지간적유전차이진행료분석，채용층차분석법，대기관상개발개치진행료정량평개．결과표명：（1）‘자협감서’적지상기관균정자색，재20개형태표기중，‘자협감서’여9개감서품충（계）최명현적형태차이시경협적색채；（2）‘자협감서’경협등지상기관적화색소함량명현고우기타품충（계），병차각기관지간적화색소함량존재명현차이，표현위협＞협병＞경；（3）사용9조인물종10개감서품충（계）중공확증출93개DNA편단，기중다태성편단위78개，점총조대수적83.8％；의거ISSR분자표기회제취류도，‘자협감서’여‘백골기룡’급‘투자263’적친연관계최근；（4）채용층차분석법대‘자협감서’적관상개발개치진행정량평개적결과표명，‘자협감서’구유교고적관상개발여이용개치．
With colorful fascicled leaves and gorgeous runners , ornamental sweet potato has excellent characteristics in resistance to poor and dry soils , which make it great application in gardening .We have found a sweet potato mu-tant, named as‘purple leaf sweet potato’ with purple leaf, petiole and stem.In this paper, inherited characters of the‘purple leaf sweet potato ’ were studied and its ornamental characters were evaluated .The main results are as follows:(1) Twenty morphological indexes were used in morphologic observation of the ‘purple leaf sweet potato ’ and other nine cultivars of sweet potato;the results showed that the above-ground organs of the ‘purple leaf sweet potato ’ are purple , which exhibited great difference from other cultivars of sweet potato , especially the color of stem and leaf.(2) The anthocyanidin content in ‘purple leaf sweet potato ’ was highest in all the experimental cul-tivars, and was high in leaves , followed by petioles and stems .(3) ISSR-PCR was used for molecular marking of all the experimental cultivars of sweet potato using 9 primers for amplification , 93 DNA fragments were amplified , which contained 78 polymorphic fragments , 83.8%of the total fragments;cluster analysis showed that ‘purple leaf sweet potato ’ had the closest genetic relationship to ‘Baiguqilong ’ and ‘Yuzi263 ’ .( 4 ) Analytic hierarchy process was used to evaluate the ornamental characters of ‘purple leaf sweet potato ’ , the results suggested that the ornamental value of ‘purple leaf sweet potato ’ is good .
