CAJ | 학술논문

采用自催化乙醇法对蔗渣原料进行预处理，并通过傅里叶变换红外光谱、扫描电镜、X射线衍射仪对预处理样品进行分析，然后进行酶解．结果表明：蔗渣原料经乙醇预处理后，有大量木质素溶出和半纤维素水解；预处理样品的相对结晶度提高了32．97％；蔗渣纤维表面碎化，细小纤维暴露出来，极大地提高了酶解效率；当温度为195℃、乙醇体积分数为40％、保温时间为30 min时，蔗渣原料木素去除率为57．97％，此时100 g蔗渣原料经此预处理所得预处理液中的木糖量为8．59 g，占原料中总木糖的35．16％，预处理样经酶解，所得酶解液中葡萄糖含量为40．29 g，占原料中总葡萄糖的92．15％；初步实现了蔗渣原料中半纤维素和纤维素的逐步分离，同时得到大量乙醇木素；最优酶用量为10 FPU/g （以每克固体浆料计），固液比为1∶40（g/mL）．
채용자최화을순법대자사원료진행예처리，병통과부리협변환홍외광보、소묘전경、X사선연사의대예처리양품진행분석，연후진행매해．결과표명：자사원료경을순예처리후，유대량목질소용출화반섬유소수해；예처리양품적상대결정도제고료32．97％；자사섬유표면쇄화，세소섬유폭로출래，겁대지제고료매해효솔；당온도위195℃、을순체적분수위40％、보온시간위30 min시，자사원료목소거제솔위57．97％，차시100 g자사원료경차예처리소득예처리액중적목당량위8．59 g，점원료중총목당적35．16％，예처리양경매해，소득매해액중포도당함량위40．29 g，점원료중총포도당적92．15％；초보실현료자사원료중반섬유소화섬유소적축보분리，동시득도대량을순목소；최우매용량위10 FPU/g （이매극고체장료계），고액비위1∶40（g/mL）．
Sugarcane bagasse was pretreated via the self-catalyzed ethanol delignification , and the pretreated sam-ples were analyzed by means of FT-IR, SEM and XRD , and then the corresponding enzymatic hydrolysis was made.The results show that (1) the pretreatment makes some lignin and hemicellulose hydrolyzed and the relative crystallinity of pretreated samples increases by 32.97%;(2) after the treatment, the surface of bagasse fibres is fragmented and fine fibres are exposed with a result that the efficiency of enzymatic hydrolysis is greatly improved ;(3) when the pretreatment is performed at the temperature of 195℃with a volume fraction of ethanol being 40%and warmth remaining for 30 min, 57.97% of lignin in the solid material are removed , and 8.59 g xylose is obtained from pre-hydrolyzate, which accounts for 35.16%of total xylose in the raw bagasse of 100 g;(4) during the subsequent enzymatic hydrolysis , 40 .29 g glucose is obtained , which accounts for 92 .15%of total glucose in the raw bagasse;(5) the gradual separation of hemicellulose and cellulose in raw material is preliminarily achieved and at the same time a large amount of ethanol lignin is obtained;and (6) the optimal enzyme dosage is 10 FPU/g and the solid-to-liquid ratio ( dry substrate ) is 1∶40 ( g/mL) .