CAJ | 학술논문

目的：观察脂联素受体（APNR）在小鼠脑缺血模型中的表达，有助于对脂联素作用机制的研究。方法60只健康雄性CD-1小鼠行短暂性大脑中动脉栓塞术，分别于缺血前、再灌注后1、4 h、1、3及7 d时处死取脑，每个时间点12只。用PT-PCR、Western blot和免疫组织化学的方法观察小鼠脑缺血再灌注后各时间点脑内APNR蛋白和mRNA表达。结果与缺血前比较，再灌注后4 h、1、3、7 d缺血侧脑组织中APNR1蛋白和mRNA表达量增高（P＜0．05，P＜0．01），缺血中心区和缺血周边区APNR1蛋白表达量相似；脑血管内皮细胞、星形胶质细胞和神经元细胞中均有APNR1的表达。各时间点小鼠缺血前后的脑组织均未见APNR2蛋白和mRNA表达。结论缺血再灌注损伤后，APNR1在缺血脑组织中表达上调，脂联素可能通过APNR1的介导发挥脑保护作用。
목적：관찰지련소수체（APNR）재소서뇌결혈모형중적표체，유조우대지련소작용궤제적연구。방법60지건강웅성CD-1소서행단잠성대뇌중동맥전새술，분별우결혈전、재관주후1、4 h、1、3급7 d시처사취뇌，매개시간점12지。용PT-PCR、Western blot화면역조직화학적방법관찰소서뇌결혈재관주후각시간점뇌내APNR단백화mRNA표체。결과여결혈전비교，재관주후4 h、1、3、7 d결혈측뇌조직중APNR1단백화mRNA표체량증고（P＜0．05，P＜0．01），결혈중심구화결혈주변구APNR1단백표체량상사；뇌혈관내피세포、성형효질세포화신경원세포중균유APNR1적표체。각시간점소서결혈전후적뇌조직균미견APNR2단백화mRNA표체。결론결혈재관주손상후，APNR1재결혈뇌조직중표체상조，지련소가능통과APNR1적개도발휘뇌보호작용。
Objective To study the mechanism of adiponectin receptor(APNR) in protecting mouse brain by observing its expression in a mouse transient cerebral ischemia model .Methods Sixty CD-1 mice that underwent tMCAO were included in this study .Their brain tissue samples were taken before ischemia .Expression of APNR in mouse brain tissue samples taken before ischemia , and at 1 and 4 h ,and on days 3 and 7 after reperfusion was detected by RT-PCR ,Western blot and immunohistochemistry ,respectively .Results The expression level of APNR1 protein and mRNA was significantly higher in ischemic tissue at 4 h and on days 3 and 7 after reperfusion than before ischemia(P<0 .05 ,P<0 .01) .The expression level of APNR1 protein was similar in central is-chemic area and peripheral ischemic area .APNR1 was expressed in endothelial cells ,astrocytes and neurons .APNR2 was not expressed in brain tissue before and after ischemia .Conclusion The expression of APNR1 is up-regulated in ischemic brain tissue following ischemia-reperfusion inju-ry .Adiponectin may exert its brain protective effect via APNR1 .