CAJ | 학술논문

目的：探讨高压氧对移植干细胞在梗死心肌组织局部的炎性状况。方法选择SD大鼠65只，随机分为假手术组5只，心肌梗死组15只，干细胞移植组15只，高压氧组15只，高压氧＋干细胞移植组（联合组）15只。建立心肌梗死大鼠模型，模型建立后第1天进行高压氧治疗30d。心肌梗死后第7天进行人脐带华通胶间充质干细胞移植，分别于心肌梗死前、心肌梗死后1、7、14、28d采集血清，ELISA法检测血清TNF-a、白细胞介素1β（IL-1β）、IL-6水平，采用EdU染色法，标记人脐带华通胶间充质干细胞，免疫荧光法检测移植细胞存活及增殖率。结果除外联合组14d时IL-6，联合组3、14、28d时TNF-a、IL-6和IL-1β较心肌梗死组、高压氧组、干细胞移植组明显降低（P＜0．05）；与干细胞移植组比较，联合组干细胞移植后7、21d心肌组织局部干细胞数量及增殖率明显增高（80．6％vs55．0％，79．1％vs34．3％，P＜0．05）。结论高压氧能够显著降低梗死心肌组织中的炎性因子水平，有效改善移植干细胞生存的微环境，提高移植干细胞在受损心肌局部的生存及增殖率。
목적：탐토고압양대이식간세포재경사심기조직국부적염성상황。방법선택SD대서65지，수궤분위가수술조5지，심기경사조15지，간세포이식조15지，고압양조15지，고압양＋간세포이식조（연합조）15지。건립심기경사대서모형，모형건립후제1천진행고압양치료30d。심기경사후제7천진행인제대화통효간충질간세포이식，분별우심기경사전、심기경사후1、7、14、28d채집혈청，ELISA법검측혈청TNF-a、백세포개소1β（IL-1β）、IL-6수평，채용EdU염색법，표기인제대화통효간충질간세포，면역형광법검측이식세포존활급증식솔。결과제외연합조14d시IL-6，연합조3、14、28d시TNF-a、IL-6화IL-1β교심기경사조、고압양조、간세포이식조명현강저（P＜0．05）；여간세포이식조비교，연합조간세포이식후7、21d심기조직국부간세포수량급증식솔명현증고（80．6％vs55．0％，79．1％vs34．3％，P＜0．05）。결론고압양능구현저강저경사심기조직중적염성인자수평，유효개선이식간세포생존적미배경，제고이식간세포재수손심기국부적생존급증식솔。
Objective To study the effect of hyperbaric oxygen on local inflammation in infracted heart tissue of rats following MSC transplantation .Methods Seventy SD rats were randomly di-vided into sham operation group (n=5) ,myocardial infarction (MI) group (n= 15) ,MSC trans-plantation group (n=15) ,hyperbaric oxygen group (n=15) ,hyperbaric oxygen plus MSC trans-plantation group (combination group ,n=15) .One day after a rat MI model was established ,the rats received hyperbaric oxygen treatment for 30 days .Human umbilical cord Wharton jelly MSC were transplanted into the rats on day 7 after MI .Serum levels of TNF-α,IL-1β and IL-6 were measured by ELISA before MI and on days 1 ,7 ,14 and 28 after MI .Human Wharton's jelly MSC were labelled with EdU .Proliferation and survival rates of MSC were assayed using immunofluo-rescence method .Results The serum levels of TNF-α,IL-6 and IL-1βwere significantly lower in combination group than in MI group ,hyperbaric oxygen group ,MSC transplantation group on days 3 ,14 and 28 after MI (P<0 .05) .The number and proliferation rate of MSC were significant-ly higher in combination group than in MSC transplantation group on days 7 and 21 after MSC transplantation (80 .6% vs 55 .0% ,79 .1% vs 34 .3% ,P<0 .05) .Conclusion Hyperbaric oxygen can significantly improve the microenrionment ,survival and proliferation rate of transplanted MSC in infarcted myocardial tissue by reducing the inflammation factors level .