中华老年心脑血管病杂志
中華老年心腦血管病雜誌
중화노년심뇌혈관병잡지
CHINESE JOURNAL OF GERIATRIC CARDIOVASCULAR AND CEREBROVASCULAR DISEASES
2014年
10期
1073-1077
,共5页
刘宏博%周军利%王小闯%薛嘉虹
劉宏博%週軍利%王小闖%薛嘉虹
류굉박%주군리%왕소틈%설가홍
脂多糖类%间质干细胞%心肌梗死%多配体蛋白聚糖-4
脂多糖類%間質榦細胞%心肌梗死%多配體蛋白聚糖-4
지다당류%간질간세포%심기경사%다배체단백취당-4
lipopolysaccharides%mesenchymal stem cells%myocardial infarction%syndecan-4
目的：研究脂多糖预处理骨髓间充质干细胞（MSC）后治疗急性心肌梗死（AMI）大鼠的机制。方法将大鼠MSC分为对照1组、脂多糖预处理为预处理1组、Toll样受体4封闭为封闭1组，定量分析多配体蛋白聚糖-4（syn-decan-4）mRNA和蛋白表达。45只大鼠建立AMI后分别植入上述各组细胞，依次为对照2组、预处理2组和封闭2组，10周后测大鼠心功能指标；另30只AMI大鼠中，将MSC转染syndecan-4过表达质粒为过表达2组，沉默MSC syndecan-4基因为联合组，每组15只。MTT法测细胞增殖，光镜下观察大鼠新生血管密度，心肌梗死面积，计算死亡率。结果与对照1组和封闭1组比较，预处理1组MSC中syndecan-4基因和蛋白表达显著上升（P＝0.000）。与对照2组和封闭2组比较，预处理2组大鼠各项心功能指标变化明显（P＜0．01）。过表达2组MSC增殖率及大鼠新生血管密度较对照2组升高（ P＝0．009）。联合组较对照2组和预处理2组M SC增殖率、心肌梗死面积[（31．2±3．0）％ vs（26．8±2．9）％和（19．7±2．5）％，P＝0．013]和死亡率（20.0％ vs13．3％ vs20．0％，P＝0.000）增高。结论脂多糖预处理能促进MSC中syndecan-4的表达，增强MSC移植在AMI中的治疗效果。
目的：研究脂多糖預處理骨髓間充質榦細胞（MSC）後治療急性心肌梗死（AMI）大鼠的機製。方法將大鼠MSC分為對照1組、脂多糖預處理為預處理1組、Toll樣受體4封閉為封閉1組，定量分析多配體蛋白聚糖-4（syn-decan-4）mRNA和蛋白錶達。45隻大鼠建立AMI後分彆植入上述各組細胞，依次為對照2組、預處理2組和封閉2組，10週後測大鼠心功能指標；另30隻AMI大鼠中，將MSC轉染syndecan-4過錶達質粒為過錶達2組，沉默MSC syndecan-4基因為聯閤組，每組15隻。MTT法測細胞增殖，光鏡下觀察大鼠新生血管密度，心肌梗死麵積，計算死亡率。結果與對照1組和封閉1組比較，預處理1組MSC中syndecan-4基因和蛋白錶達顯著上升（P＝0.000）。與對照2組和封閉2組比較，預處理2組大鼠各項心功能指標變化明顯（P＜0．01）。過錶達2組MSC增殖率及大鼠新生血管密度較對照2組升高（ P＝0．009）。聯閤組較對照2組和預處理2組M SC增殖率、心肌梗死麵積[（31．2±3．0）％ vs（26．8±2．9）％和（19．7±2．5）％，P＝0．013]和死亡率（20.0％ vs13．3％ vs20．0％，P＝0.000）增高。結論脂多糖預處理能促進MSC中syndecan-4的錶達，增彊MSC移植在AMI中的治療效果。
목적：연구지다당예처리골수간충질간세포（MSC）후치료급성심기경사（AMI）대서적궤제。방법장대서MSC분위대조1조、지다당예처리위예처리1조、Toll양수체4봉폐위봉폐1조，정량분석다배체단백취당-4（syn-decan-4）mRNA화단백표체。45지대서건립AMI후분별식입상술각조세포，의차위대조2조、예처리2조화봉폐2조，10주후측대서심공능지표；령30지AMI대서중，장MSC전염syndecan-4과표체질립위과표체2조，침묵MSC syndecan-4기인위연합조，매조15지。MTT법측세포증식，광경하관찰대서신생혈관밀도，심기경사면적，계산사망솔。결과여대조1조화봉폐1조비교，예처리1조MSC중syndecan-4기인화단백표체현저상승（P＝0.000）。여대조2조화봉폐2조비교，예처리2조대서각항심공능지표변화명현（P＜0．01）。과표체2조MSC증식솔급대서신생혈관밀도교대조2조승고（ P＝0．009）。연합조교대조2조화예처리2조M SC증식솔、심기경사면적[（31．2±3．0）％ vs（26．8±2．9）％화（19．7±2．5）％，P＝0．013]화사망솔（20.0％ vs13．3％ vs20．0％，P＝0.000）증고。결론지다당예처리능촉진MSC중syndecan-4적표체，증강MSC이식재AMI중적치료효과。
Objective To study whether lipopolysaccharide (LPS ) preconditioning improves the therapeutic effect of MSC transplantation in a rat model of acute myocardial infarction (AMI) . Methods MSC were divided into control group 1 ,LPS preconditioning group ,and Toll-like recep-tor 4 (TLR4) blocking group .Expression of syndecan-4 mRNA and protein in MSC was detected by quantitative analysis .The MSC were then divided into control group 2 ,LPS preconditioning group 2 ,and TLR4 blocking group 2 .Overexpressed plasmids in MSC-transfected syndecan 4 served as an overexpression group and silence syndecan 4 in MSC served as a combination group . Proliferation of MSC was assayed by MTT .The MSC were implanted into AMI rats .Angiogene-sis density was detected .Results The expression level of syndecan 4 mRNA and protein in MSC was significantly higher in LPS preconditioning group than in control group 1 and TLR4 blocking group 1 (P=0 .000) .The cardiac function was significantly better in LPS preconditioning group than in control group 2 and TLR4 blocking group 2 (P<0 .01) .The proliferation rate of MSC and angiogenesis density were significantly higher in overexpression group than in control group 2 and TLR4 blocking group 2 (P=0 .009) .The proliferation rate of MSC was lower while the MI size was larger and the mortality was higher in combination group than in other groups (F=16 .739 , P=0 .013;20 .0% vs 13 .3% vs 20 .0% ,χ2 =13 .755 ,P=0 .000) .Conclusion LPS preconditioning up-regulates the syndecan 4 expression in MSC ,thus improving the therapeutic effect of MSC transplantation on AMI .
