CAJ | 학술논문

目的：探讨激动β3肾上腺素受体（β3-AR）对载脂蛋白E基因敲除（apoE-/-）小鼠血糖、胰岛素和胰腺血管紧张素Ⅱ受体（ATR）表达水平的影响。方法选用10周龄C57BL/6J小鼠10只为对照组（A 组），另选10周龄apo E-/-小鼠50只，高脂饮食至36周龄时随机分为高脂模型组（B组）、阿托伐他汀阳性药物对照组（C组）、β3-A R激动剂小剂量组（D组）、β3-A R激动剂大剂量组（E组）和β3-A R拮抗剂组（F组），干预12周。48周时检测小鼠血糖和胰岛素水平；采用实时定量PCR和Western blot检测各组小鼠AT1 R和AT2 R表达水平。结果与A组比较，B组血糖、胰岛素明显升高，AT1 R明显上调，AT2 R明显下调（P＜0．01）；与B组比较，D组、E组血糖、胰岛素明显降低，AT1 R明显下调，AT2 R明显上调（P＜0．01）。结论长期应用β3-AR激动剂通过下调apoE-/-老年高脂小鼠胰腺AT1 R表达和上调AT2 R表达，β3-AR与AT1 R和AT2 R存在交互作用，且与改善糖代谢紊乱有关。
목적：탐토격동β3신상선소수체（β3-AR）대재지단백E기인고제（apoE-/-）소서혈당、이도소화이선혈관긴장소Ⅱ수체（ATR）표체수평적영향。방법선용10주령C57BL/6J소서10지위대조조（A 조），령선10주령apo E-/-소서50지，고지음식지36주령시수궤분위고지모형조（B조）、아탁벌타정양성약물대조조（C조）、β3-A R격동제소제량조（D조）、β3-A R격동제대제량조（E조）화β3-A R길항제조（F조），간예12주。48주시검측소서혈당화이도소수평；채용실시정량PCR화Western blot검측각조소서AT1 R화AT2 R표체수평。결과여A조비교，B조혈당、이도소명현승고，AT1 R명현상조，AT2 R명현하조（P＜0．01）；여B조비교，D조、E조혈당、이도소명현강저，AT1 R명현하조，AT2 R명현상조（P＜0．01）。결론장기응용β3-AR격동제통과하조apoE-/-노년고지소서이선AT1 R표체화상조AT2 R표체，β3-AR여AT1 R화AT2 R존재교호작용，차여개선당대사문란유관。
Objective To study the effect ofβ3 adrenoceptor (β3-AR)activation on serum blood glu-cose and insulin level and expression of pancreatic angiotensin receptor (ATR) in apoE/mice . Methods Ten wild-type C57BL/6J mice at the age of 10 weeks served as a normal control group (group A)and 50 apoE/mice at the age of 10 weeks fed with high fat diet to 36 weeks old were randomly divided into hyperlipidaemia model group (group B ) ,atorvastatin treatment group (group C) ,low β3-AR agonist dose group (group D) ,high β3-AR agonist dose group (group E) andβ3-AR antagonist group (group F) ,10 in each group .The mice were treated withβ3-AR ago-nist and antagonist for 12 weeks .Serum blood glucose and insulin levels in 48 weeks old mice were measured using an automatic biochemical detector .Expression of AT1 R and AT2 R in pancre-atic tissues of mice was detected by RT-PCR and Western blot ,respectively .Results The serum blood glucose and insulin levels were significantly higher ,the expression level of AT1 R was sig-nificantly higher while that of AT2R was significantly lower in group B than in group A (P<0.01) .The serum blood glucose and insulin levels were significantly lower ,the expression level of AT1 R was significantly lower while that of AT2 R was significantly higher in groups D and E than in group B ( P<0 .01) .Conclusion Long-term use of β3-AR agonist can improve blood glucose metabolism in apoE/mice by down-regulating the AT1 R expression and up-regulating the AT2 R expression ,indicating that β3-AR interacts with AT1 R and AT2 R .