CAJ | 학술논문

目的：对来自贵州省的8例空肠弯曲菌病疑似病例进行病原学诊断与分子流行病学特征分析，为病例的确诊和贵州省空肠弯曲菌病疫情的预防和控制提供科学依据。方法对8例疑似病例粪便和血液标本进行细菌分离，采用传统方法和多重PCR方法对空肠弯曲菌可疑菌株进行鉴定，应用脉冲场凝胶电泳分型技术（PFGE）对空场弯曲菌分离株进行分析。结果8例空肠弯曲菌病疑似病例标本均分离出空肠弯曲菌可疑菌株，PCR扩增出空肠弯曲菌预期大小的目的基因片段，PF-GE显示8株菌经 SamI酶切后产生7～10条DNA条带，聚类分析显示，8株菌株分为7个PFGE带型，8株菌的相似性＞50％，其中GZ201004和GZ201005两株腹泻病例来源分离株相似性为100％，血培养血液标本分离株GZ201201与粪便标本分离株 GZ201007相似性为66．7％。结论8例空肠弯曲菌病疑似病例均检出空肠弯曲菌，PFGE 显示 GZ201004和GZ201005两株为同一来源，但8例病例来源菌株存在PFGE带型多态性，该研究结果为病例的确诊和疫情的预防和控制提供了科学依据。
목적：대래자귀주성적8례공장만곡균병의사병례진행병원학진단여분자류행병학특정분석，위병례적학진화귀주성공장만곡균병역정적예방화공제제공과학의거。방법대8례의사병례분편화혈액표본진행세균분리，채용전통방법화다중PCR방법대공장만곡균가의균주진행감정，응용맥충장응효전영분형기술（PFGE）대공장만곡균분리주진행분석。결과8례공장만곡균병의사병례표본균분리출공장만곡균가의균주，PCR확증출공장만곡균예기대소적목적기인편단，PF-GE현시8주균경 SamI매절후산생7～10조DNA조대，취류분석현시，8주균주분위7개PFGE대형，8주균적상사성＞50％，기중GZ201004화GZ201005량주복사병례래원분리주상사성위100％，혈배양혈액표본분리주GZ201201여분편표본분리주 GZ201007상사성위66．7％。결론8례공장만곡균병의사병례균검출공장만곡균，PFGE 현시 GZ201004화GZ201005량주위동일래원，단8례병례래원균주존재PFGE대형다태성，해연구결과위병례적학진화역정적예방화공제제공료과학의거。
Eight patients with suspected cases of C .jejuni were etiologically diagnosed and analyzed in this study to pro-vide scientific basis for the confirmation of the cases of human campylobacteriosis in Guizhou Province ,China .Blood or feces of 8 suspected patients were employed to isolate bacteria strains .Conventional and multi-PCR techniques were applied to identify suspicious bacteria strains .The C .jejuni strains were analyzed by using Pulsed Field Gel Electrophoresis (PFGE) .Suspicious strains of C .jejuni were isolated from all the 8 suspected patients of campylobacteriosis and anticipated genes fragment were detected with multi-PCR .With the digestion of restriction enzyme SmaI ,the 8 C .jejuni strains were divided into 7 PFGE pat-terns with 7-10 DNA bands .Cluster analysis showed that the gross similarity of 8 strains of C . jejuni was more than 50% . The similarity of PFGE patterns between strain GZ201004 and GZ201005 from diarrhea patients was as high as 100% ,while the similarity of strain GZ201201 and GZ201007 was 66 .7% .Moreover ,C . jejuni were detected from all the suspected pa-tients of campylobacteriosis .PFGE results indicated that strains GZ201004 and GZ201005 were from the same source ,while all the 8 isolates showed PFGE polymorphism .