CAJ | 학술논문

目的：了解环洞庭湖区主要活禽批发市场低致病性禽流感病毒的分布和流行情况，为该地区活禽批发市场低致病性禽流感的防控措施提供一些依据。方法对在活禽批发市场上采集的拭子样品先由尿囊腔接种SPF鸡胚，然后用血凝试验（HA）测定所收集的尿囊液，对HA测定有滴度的再进行血凝抑制试验（HI）和RT-PCT方法相结合鉴定病毒的亚型。结果2009-2011年冬春季节，我们在环洞庭湖地区主要活禽批发市场上共分离到627株低致病性禽流感病毒，总的分离率为22．2％，其中鸭拭子低致病性禽流感病毒的分离率最高，达到24．6％，其次是鸡拭子，达到21．5％，鹅拭子为11％。在所监测的5家活禽批发市场中共分离到 H3、H4、H6、H9、H10型和H11等6种亚型的低致病性禽流感病毒，其中 H9、H6和 H4亚型比较高，其分离率分别可达到11％、6．3％和3．4％。结论活禽批发市场中低致病性禽流感隐性带毒的情况普遍存在，其发生禽流感疫情的威胁较大。建议整顿规范市场经营管理，特别是要建立活禽交易市场定期休市制度。
목적：료해배동정호구주요활금비발시장저치병성금류감병독적분포화류행정황，위해지구활금비발시장저치병성금류감적방공조시제공일사의거。방법대재활금비발시장상채집적식자양품선유뇨낭강접충SPF계배，연후용혈응시험（HA）측정소수집적뇨낭액，대HA측정유적도적재진행혈응억제시험（HI）화RT-PCT방법상결합감정병독적아형。결과2009-2011년동춘계절，아문재배동정호지구주요활금비발시장상공분리도627주저치병성금류감병독，총적분리솔위22．2％，기중압식자저치병성금류감병독적분리솔최고，체도24．6％，기차시계식자，체도21．5％，아식자위11％。재소감측적5가활금비발시장중공분리도 H3、H4、H6、H9、H10형화H11등6충아형적저치병성금류감병독，기중 H9、H6화 H4아형비교고，기분리솔분별가체도11％、6．3％화3．4％。결론활금비발시장중저치병성금류감은성대독적정황보편존재，기발생금류감역정적위협교대。건의정돈규범시장경영관리，특별시요건립활금교역시장정기휴시제도。
The distribution and prevalence of low pathogenic avian influenza virus in major live poultry wholesale markets around the Dongting Lake region ,China were investigated in our study to propose prevention and control measures on low pathogenic avian flu in the area of live poultry wholesale market .The samples were injected to SPF chicken embryos by allanto-ic cavity ,and then the allantoic fluid were harvested and used for hemagglutination (HA) .If it was positive by HA ,subtypes of the virus would be determined by hemagglutination inhibition (HI) and RT-PCT .We isolated 627 low pathogenic avian in-fluenza viruses in major live poultry wholesale market around Dongting Lake region systematically in winter and spring during 2009-2011 ,and the total separation rate was 22 .2% .The duck swab separation rate of low pathogenic avian influenza was the highest ,which was 24 .6% ,and the following was chicken swab that reached 21 .5% ,and the goose swab separation rate was 11% .We isolated 6 HA subtypes including H3 ,H4 ,H6 ,H9 ,H10 ,and H11 in every live poultry wholesale market ,and the separation rate of H9 ,H6 and H4 subtypes was relatively high ,which could reach 11% ,6 .3% and 3 .4% ,respectively . Those results indicated that recessive infection of low pathogenic avian influenza virus was serious in live poultry wholesale mar-ket around the Dongting Lake area ,and it was a great threat to the occurrence of avian flu .