河北医科大学学报
河北醫科大學學報
하북의과대학학보
JOURNAL OF HEBEI MEDICAL UNIVERSITY
2014年
10期
1166-1169
,共4页
孙静娜%刘青松%刘泽世%赵帅%王国欣%张征
孫靜娜%劉青鬆%劉澤世%趙帥%王國訢%張徵
손정나%류청송%류택세%조수%왕국흔%장정
鲍氏不动杆菌%抗药性,多药%基因表达
鮑氏不動桿菌%抗藥性,多藥%基因錶達
포씨불동간균%항약성,다약%기인표체
acinetobacter baumannii%drug resistance,multiple%gene expression
目的:观察多重耐药及泛耐药鲍曼不动杆菌主动外排泵在耐药方面的作用和外排泵基因表达情况。方法通过比较加入羰基氰氯苯(carbonyl cyanldem-chlorophenyl hydrazone,CCCP)泵抑制剂前后,96株鲍曼不动杆菌对四环素、诺氟沙星、庆大霉素、头孢噻肟4种药物最小抑菌浓度(minimum inhibitory concentration,MIC)的变化,筛选出34株外排泵表型阳性的鲍曼不动杆菌。用聚合酶链反应扩增对34株菌进行外排泵蛋白基因adeB的检测和分析,并测序。结果最终筛选出对4种抗生素MIC值均降低4倍及以上菌34株(35.42%),34株外排泵表型阳性的鲍曼不动杆菌检测到 adeB基因33株,检出阳性率为97.06%。对33株鲍曼不动杆菌的外排泵基因 adeB进行测序,经比对所测序列与基因库(Genebank)中序列同源性为100.00%。结论泵抑制剂CCCP对于逆转鲍曼不动杆菌对四环素、诺氟沙星、庆大霉素、头孢噻肟4种抗菌药物的耐药性起到重要作用,主动外排泵基因是鲍曼不动杆菌发生多重耐药的重要因素。
目的:觀察多重耐藥及汎耐藥鮑曼不動桿菌主動外排泵在耐藥方麵的作用和外排泵基因錶達情況。方法通過比較加入羰基氰氯苯(carbonyl cyanldem-chlorophenyl hydrazone,CCCP)泵抑製劑前後,96株鮑曼不動桿菌對四環素、諾氟沙星、慶大黴素、頭孢噻肟4種藥物最小抑菌濃度(minimum inhibitory concentration,MIC)的變化,篩選齣34株外排泵錶型暘性的鮑曼不動桿菌。用聚閤酶鏈反應擴增對34株菌進行外排泵蛋白基因adeB的檢測和分析,併測序。結果最終篩選齣對4種抗生素MIC值均降低4倍及以上菌34株(35.42%),34株外排泵錶型暘性的鮑曼不動桿菌檢測到 adeB基因33株,檢齣暘性率為97.06%。對33株鮑曼不動桿菌的外排泵基因 adeB進行測序,經比對所測序列與基因庫(Genebank)中序列同源性為100.00%。結論泵抑製劑CCCP對于逆轉鮑曼不動桿菌對四環素、諾氟沙星、慶大黴素、頭孢噻肟4種抗菌藥物的耐藥性起到重要作用,主動外排泵基因是鮑曼不動桿菌髮生多重耐藥的重要因素。
목적:관찰다중내약급범내약포만불동간균주동외배빙재내약방면적작용화외배빙기인표체정황。방법통과비교가입탄기청록분(carbonyl cyanldem-chlorophenyl hydrazone,CCCP)빙억제제전후,96주포만불동간균대사배소、낙불사성、경대매소、두포새우4충약물최소억균농도(minimum inhibitory concentration,MIC)적변화,사선출34주외배빙표형양성적포만불동간균。용취합매련반응확증대34주균진행외배빙단백기인adeB적검측화분석,병측서。결과최종사선출대4충항생소MIC치균강저4배급이상균34주(35.42%),34주외배빙표형양성적포만불동간균검측도 adeB기인33주,검출양성솔위97.06%。대33주포만불동간균적외배빙기인 adeB진행측서,경비대소측서렬여기인고(Genebank)중서렬동원성위100.00%。결론빙억제제CCCP대우역전포만불동간균대사배소、낙불사성、경대매소、두포새우4충항균약물적내약성기도중요작용,주동외배빙기인시포만불동간균발생다중내약적중요인소。
Objective To investigate efflux pump drug-resistance and gene expression in multidrug resistant and pandrug resistant Acinetobacter baumannii.Methods The multidrug resistant Acinetobacter baumannii efflux pump phenotypes were detected by joining carbonyl cyanidem-chloropheny hydrazone (CCCP)pump inhibitors,the minimum inhibitory concentration (MIC)variation was observed in 9 6 strains of Acinetobacter baumannii resistant to tetracycline, norfloxacin,gentamicin,cefotaxime,34 strains of efflux phenotype positive Acinetobacter baumannii were screened out.The efflux pump protein gene adeB in 34 Acinetobacter baumannii were detected and sequenced with the polymerase chain reaction amplification.Results The initial selection involoed 34 strains (35.42%)whose MIC reduced by 4 times and above.In 34 stains of efflux pump phenotype positive Acinetobacter baumannii,33 strains were detected adeB gene,the positive rate was 97.06%.When the 33 strains Aacinetobacter baumannii adeB efflux pump gene were sequenced,the sequence homology was 100.00% compared in Genebank.Conclusion Pump inhibitor CCCP plays an important role in the reversal of Acinetobacter baumannii resistant to tetracycline,norfloxacin,gentamicin and cefotaxime,active efflux pump genes play an important role in multidrug resistant Acinetobacter baumannii.