CAJ | 학술논문

目的：研究人卵巢颗粒细胞叉头框（forkhead box，Fox）基因和卵泡刺激素受体（follicle stimulating hormone receptor，FSHR）的表达及相关性。方法接受体外受精-胚胎移植（in vitro fertilization-embryo transfer， IVF-ET）不孕症患者126例，按照妊娠结果分为妊娠组63例，未妊娠组63例。回顾性分析取卵日成熟卵泡壁颗粒细胞 FoxO1、FoxO3a 和 FSHR mRNA 的表达，并对 FoxO1、FoxO3a、FSHR 之间及与血清促黄体生成素（luteotropic hormone，LH）、雌二醇（estradiol，E2）、孕酮（progesterone，P）进行相关性分析。结果妊娠组血清 E2水平高于未妊娠组（P＜0．01）；妊娠组优质胚胎数多于未妊娠组（P＜0．01）；2组 LH、P、获卵数差异无统计学意义（P＞0．05）。妊娠组 FoxO1 mRNA表达高于未妊娠组（P＜0．01）；2组 FoxO3a及 FSHR mRNA差异无统计学意义（P＞0．05）。FoxO1和 FoxO3a与FSHR的相关性系数分别为0．881、0．999（P＜0．01i）。结论 FoxO1和FoxO3a参与卵母细胞质量的调节，其功能与 FSHR有密切关系。
목적：연구인란소과립세포차두광（forkhead box，Fox）기인화란포자격소수체（follicle stimulating hormone receptor，FSHR）적표체급상관성。방법접수체외수정-배태이식（in vitro fertilization-embryo transfer， IVF-ET）불잉증환자126례，안조임신결과분위임신조63례，미임신조63례。회고성분석취란일성숙란포벽과립세포 FoxO1、FoxO3a 화 FSHR mRNA 적표체，병대 FoxO1、FoxO3a、FSHR 지간급여혈청촉황체생성소（luteotropic hormone，LH）、자이순（estradiol，E2）、잉동（progesterone，P）진행상관성분석。결과임신조혈청 E2수평고우미임신조（P＜0．01）；임신조우질배태수다우미임신조（P＜0．01）；2조 LH、P、획란수차이무통계학의의（P＞0．05）。임신조 FoxO1 mRNA표체고우미임신조（P＜0．01）；2조 FoxO3a급 FSHR mRNA차이무통계학의의（P＞0．05）。FoxO1화 FoxO3a여FSHR적상관성계수분별위0．881、0．999（P＜0．01i）。결론 FoxO1화FoxO3a삼여란모세포질량적조절，기공능여 FSHR유밀절관계。
Objective To explore the expression of forkhead box(FoxO)gene and follicle-stimulating hormone receptor (FSHR)in human ovarian granulosa cells and their relationship. Methods One hundred and twenty-six unfertility women treated with in vitro fertilization and embryo transplantation (IVF-ET ) were recruited.According to the pregnancy outcome,the patients were divided into pregnancy groups 63 cases and non-pregnancy group 63 cases.The expressions of FoxO1 mRNA,FoxO3a mRNA and FSHR mRNA on human ovarian granulosa cells were detected by real time fluorescence quantitative PCR.The correlation analysis of FoxO1, FoxO3a,FSHR with serum sex hormones including luteotropic hormone(LH),estradiol(E2 ), progesterone(P)were mevaluated.Results High quality embryo number and serum E2 level in pregnancy group were significantly higher than those of non-pregnancy group (P<0.01 ),but LH,P level and number of eggs showed no significant difference between two groups (P>0.05). The expression FoxO1 mRNA in pregnancy group was higher than that of non-pregnancy group (P<0.01).The expression of FoxO3a and FSHR mRNA were no statistical difference between pregnancy group and non-pregnancy group (P>0.05).The correlation coefficients of FoxO1 and FoxO3a with FSHR were 0.881,0.999 (P<0.01 ).Conclusion FoxO1 and FoxO3a genes participate in the regulation of oocyte quality and their functions have closely connected with FSHR.