CAJ | 학술논문

目的：探讨流式细胞术（FCM）结合 PCR 技术检测 TCR 基因重排在 T 细胞非霍奇金淋巴瘤（T-NHL）诊断中的作用。方法：对经组织学确诊的32例 T-NHL 的组织学形态、免疫组化、FCM 免疫表型、细针穿刺细胞学形态及 TCR 基因重排情况进行回顾性分析。对照组为18例反应性增生和1例坏死性淋巴结炎。结果：组织学诊断的32例 T-NHL 及19例对照组中，经 FCM / TCR 基因重排确诊的分别有23例和17例。 FCM /TCR 基因重排诊断与组织学诊断比较，敏感性为71．9％，特异性为89．5％，准确率为78．4％。结论：FCM 免疫表型结合 TCR 基因重排可作为诊断 T-NHL 的重要辅助手段之一，尤其对于不能取活检的，需行细针穿刺诊断的患者有较大应用价值。
목적：탐토류식세포술（FCM）결합 PCR 기술검측 TCR 기인중배재 T 세포비곽기금림파류（T-NHL）진단중적작용。방법：대경조직학학진적32례 T-NHL 적조직학형태、면역조화、FCM 면역표형、세침천자세포학형태급 TCR 기인중배정황진행회고성분석。대조조위18례반응성증생화1례배사성림파결염。결과：조직학진단적32례 T-NHL 급19례대조조중，경 FCM / TCR 기인중배학진적분별유23례화17례。 FCM /TCR 기인중배진단여조직학진단비교，민감성위71．9％，특이성위89．5％，준학솔위78．4％。결론：FCM 면역표형결합 TCR 기인중배가작위진단 T-NHL 적중요보조수단지일，우기대우불능취활검적，수행세침천자진단적환자유교대응용개치。
Objective To study the usefulness of combined flow cytometry (FCM) and polymerasechain reaction examination for clonal TCR gene rearrangements in the diagnosis of T-cell lymphoma (T-NHL). Methods Histopathologic features, immunohistochemistry, flow cytometric immunophenotyping, cytomorphologic evaluation and TCR gene rearrangements of 32 T-NHL were reviewed retrospectively. The control cases were 18 reactive lesions and 1 histiocytic necrotizing lymphaderitis. Results Out of 32 T-NHL,23 were diagnosed as T-NHL by FCM / TCR gene rearrangements. Of 19 control group, 17 were diagnosed as reactive lesions by FCM / TCR gene rearrangements. The sensitivity, specificity and accuracy were 71.9%, 89.5% and 78.4%, respectively. Conclusions FCM / TCR gene rearrangement is a very important technique in diagnosing T-NHL. Thus, patients with fine needle aspiration cytology can be saved from having an invasive surgery.