CAJ | 학술논문

目的：寻找结核性胸膜炎特异性体液蛋白质标志物，以开发新型结核性胸膜炎实验诊断方法。方法：应用表面增强激光解析电离飞行时间质谱（SELDI-TOF-MS）技术检测5例结核性胸膜炎患者结核分枝杆菌分离株 Middlebrook 7H9液体培养早中期（7 d 与14 d）培养滤液及其血清、胸腔积液蛋白质组，并进行描述性比较分析，寻找其共有蛋白质组分。结果：培养滤液蛋白质组蛋白质种类数量远多于胸腔积液和血清蛋白质组，4例患者胸腔积液蛋白质组蛋白质种类数量多于血清蛋白质组；5例患者3类样本蛋白质组中，存在种类数量各不相同的培养滤液-胸腔积液、培养滤液-血清、血清-胸腔积液和培养滤液-胸腔积液-血清共有蛋白质，但在所有患者培养滤液、胸腔积液、血清中均存在相对分子质量为2660的蛋白质。结论：相对分子质量为2660的蛋白质具备结核性胸膜炎特异性体液蛋白质标志物的表征，但尚需进一步大样本量研究予以证实。
목적：심조결핵성흉막염특이성체액단백질표지물，이개발신형결핵성흉막염실험진단방법。방법：응용표면증강격광해석전리비행시간질보（SELDI-TOF-MS）기술검측5례결핵성흉막염환자결핵분지간균분리주 Middlebrook 7H9액체배양조중기（7 d 여14 d）배양려액급기혈청、흉강적액단백질조，병진행묘술성비교분석，심조기공유단백질조분。결과：배양려액단백질조단백질충류수량원다우흉강적액화혈청단백질조，4례환자흉강적액단백질조단백질충류수량다우혈청단백질조；5례환자3류양본단백질조중，존재충류수량각불상동적배양려액-흉강적액、배양려액-혈청、혈청-흉강적액화배양려액-흉강적액-혈청공유단백질，단재소유환자배양려액、흉강적액、혈청중균존재상대분자질량위2660적단백질。결론：상대분자질량위2660적단백질구비결핵성흉막염특이성체액단백질표지물적표정，단상수진일보대양본량연구여이증실。
Objective To find humoral protein markers to develop new experimental diagnostic methods for tuberculous pleurisy. Methods Proteomes of 7 d and 14 d culture filtrate of Mycobacterium tuberculosis growing in Middlebrook 7H9, serum and pleural effusion from five patients with tuberculous pleurisy were detected by surface-enhanced laser desorptionionization time-of-flight massspectrometry (SELDI-TOF-MS). And the data were analyzed with descriptive statistics method to observed the protein components all owned by three kinds of proteome. Results From protein species, proteins of all culture filtrate were far more than that of pleural effusion and serum while proteins of pleural effusion in four cases were more than that of serum. The kinds of common proteins between culture filtrate and pleural effusion, between culture filtrate and serum, between serum and pleural effusion, among culture filtrate and pleural effusion and serum were different. But the protein of relative molecular mass of 2 660 depending on the ratio of mass to charge existed in all samples of culture filtrate, pleural effusion and serum. Conclusion The protein of relative molecular mass of 2 660 possess the latent quality as a specific humoral protein marker for tuberculous pleurisy. But it is essential that must be further confirmed among large samples.