CAJ | 학술논문

目的：探讨骨髓间充质干细胞（MSCs）对外周血 CD8＋ T 淋巴细胞增殖的抑制作用及其机制。方法：分离获取 MSCs，并多方面对 MSCs 进行鉴定；检测 MSCs 对植物血凝素（PHA）刺激外周血单个核细胞（PBMCs）增殖的抑制作用；应用流式细胞仪技术检测 MSCs 对 PHA 诱导 CD8＋ T 细胞增殖的抑制作用；应用Transwell 方法探索 MSCs 抑制 PHA 刺激 CD8＋ T 细胞增殖作用的机制。结果：体外成功分离培养获得 MSCs；当 MSCs ∶ PBMCs≥1∶5时，MSCs 可抑制 PHA 引起的 CD8＋ T 细胞的增殖，且该作用呈浓度依赖性。Transwell 培养组（MSCs ∶ PBMCs ＝1∶1），MSCs 对 PHA 诱导的 CD8＋ T 细胞仍表现出明显的增殖抑制作用，与非 Transwell组作用相似。结论：MSCs 可通过抑制 CD8＋ T 细胞增殖而影响机体免疫反应。
목적：탐토골수간충질간세포（MSCs）대외주혈 CD8＋ T 림파세포증식적억제작용급기궤제。방법：분리획취 MSCs，병다방면대 MSCs 진행감정；검측 MSCs 대식물혈응소（PHA）자격외주혈단개핵세포（PBMCs）증식적억제작용；응용류식세포의기술검측 MSCs 대 PHA 유도 CD8＋ T 세포증식적억제작용；응용Transwell 방법탐색 MSCs 억제 PHA 자격 CD8＋ T 세포증식작용적궤제。결과：체외성공분리배양획득 MSCs；당 MSCs ∶ PBMCs≥1∶5시，MSCs 가억제 PHA 인기적 CD8＋ T 세포적증식，차해작용정농도의뢰성。Transwell 배양조（MSCs ∶ PBMCs ＝1∶1），MSCs 대 PHA 유도적 CD8＋ T 세포잉표현출명현적증식억제작용，여비 Transwell조작용상사。결론：MSCs 가통과억제 CD8＋ T 세포증식이영향궤체면역반응。
Objective To investigate the effect of human bone marrow mesenchymal stem cells (MSCs) on proliferation of CD8+ T lymphocytes and its mechanism. Methods MSCs were isolated and cultured then identified through many ways. The proliferative influence of MSCs on peripheral blood mononuclear cells (PBMCs) stimulated by PHA was investigated. The effect of MSCs on proliferation of CD8 + T lymphocytes induced by PHA was explored by flow cytometry. The possible mechanism of the inhibition effect of MSCs was investigated on the proliferation of CD8+ T cells stimulated by PHA with Transwell assay. Results MSCs were successfully harvested and cultured in vitro. MSCs suppressed the proliferation of CD8+ T cells stimulated by PHA when MSCs ∶ PBMCs ≥ 1 ∶ 5, which showed a dose-dependent manner. Strong proliferative inhibition of MSCs was presented on the CD8 + T cells induced by PHA in the group of Transwell (MSCs ∶ PBMCs = 1 ∶ 1) and the influence was similar to non-Transwell group. Conclusion MSCs can affect body immunity via suppressing the proliferation of CD8+ T cells.