CAJ | 학술논문

目的：研究别嘌呤醇对海人酸致颞叶癫痫大鼠的保护作用，为癫痫的治疗提供新的思路和方法。方法：将120只 Wistar 大鼠随机分为 sham 组、KA 癫痫模型组和别嘌呤醇组。每组大鼠随机抽取6只左额及海马植入电极。注药后立即观察非植入电极大鼠的行为学变化；24 h 后检测各组大鼠左侧海马组织丙二醛（MDA）含量、超氧化物歧化酶（SOD）酶活性；1周后描记各组植入电极大鼠脑电图并记录30 min 内癫痫发作总次数及总时间。结果：与 KA 癫痫模型组相比，别嘌呤醇组癫痫发作潜伏期显著延长（P ＜0．05），发作程度显著减轻（P ＜0．05）；MDA 含量显著降低（P ＜0．01），SOD 酶活性显著升高（P ＜0．01）；在记录30 min 时间内，别嘌呤醇组发作总时间和总次数均显著减少（P ＜0．01）。结论：别嘌呤醇在海人酸致颞叶癫痫大鼠模型中具有抗癫痫和抗氧化作用。
목적：연구별표령순대해인산치섭협전간대서적보호작용，위전간적치료제공신적사로화방법。방법：장120지 Wistar 대서수궤분위 sham 조、KA 전간모형조화별표령순조。매조대서수궤추취6지좌액급해마식입전겁。주약후립즉관찰비식입전겁대서적행위학변화；24 h 후검측각조대서좌측해마조직병이철（MDA）함량、초양화물기화매（SOD）매활성；1주후묘기각조식입전겁대서뇌전도병기록30 min 내전간발작총차수급총시간。결과：여 KA 전간모형조상비，별표령순조전간발작잠복기현저연장（P ＜0．05），발작정도현저감경（P ＜0．05）；MDA 함량현저강저（P ＜0．01），SOD 매활성현저승고（P ＜0．01）；재기록30 min 시간내，별표령순조발작총시간화총차수균현저감소（P ＜0．01）。결론：별표령순재해인산치섭협전간대서모형중구유항전간화항양화작용。
Objective To investigate the protective effect of allopurinol in kainic acid-induced epileptic rats and to explore new ideas and methods for the clinical treatment of epilepsy. Methods 120 Wistar rats were randomly divided into sham group, KA epilepsy group and allopurinol groups. Six rats of each group were randomly selected and were given electrodes into their left frontal and hippocampal regions. After injection, behavior changes were observed in all rates without electrodes. 24 h later, MDA level and SOD enzymatic activity of the left hippocampi were measured. One week later, the EEGs were recorded in rates with electrode, as well as total time of seizures /30 min and numbers of seizures / 30 min. Results Compared with the KA model group, latency period of the epilepsy in the allopurinol group was longer (P < 0.05) and the extent was lighter (P < 0.05); the MDA level was significantly lower (P < 0.01), the SOD enzymatic activity was significantly higher (P < 0.01). The total time of seizures / 30min and numbers of seizures / 30 min in allopurinol group reduced significantly (P < 0.01). Conclusion Allopurinol has potential antiepileptic and antioxidative activities in kainic acid-induced epileptic rats.