CAJ | 학술논문

目的：观察辛伐他汀对肺纤维化大鼠肺组织血管新生及部分调控基因表达的影响。方法：选取 SD大鼠96只，随机分为4组：空白组（A 组）、模型组（B 组）、泼尼松组（C 组）、辛伐他汀组（D 组），建模后，于7、14、28 d 随机处死8只，取肺组织为检测标本，消化法测定羟脯氨酸（HYP），免疫组化法测血管新生微血管密度（MVD）及血管内皮生长因子（VEGF）、血小板第四因子（PF4）蛋白的表达，RT-PCR 法测 VEGF 及 PF4 mRNA 表达。结果：（1）C、D 组 HYP 较 B 组减轻（均 P ＜0．01）；（2）B、C、D 组 MVD 及 VEGF 表达高于 A 组，PF4表达低于 A 组（均 P ＜0．01）；D 组 MVD 及 VEGF 表达低于 B 组，PF4表达高于 B 组（均 P ＜0．05）。结论：辛伐他汀有改善纤维化作用，其机制可能与其调节肺组织内 VEGF 及 PF4表达、抑制病理性血管新生有关。
목적：관찰신벌타정대폐섬유화대서폐조직혈관신생급부분조공기인표체적영향。방법：선취 SD대서96지，수궤분위4조：공백조（A 조）、모형조（B 조）、발니송조（C 조）、신벌타정조（D 조），건모후，우7、14、28 d 수궤처사8지，취폐조직위검측표본，소화법측정간포안산（HYP），면역조화법측혈관신생미혈관밀도（MVD）급혈관내피생장인자（VEGF）、혈소판제사인자（PF4）단백적표체，RT-PCR 법측 VEGF 급 PF4 mRNA 표체。결과：（1）C、D 조 HYP 교 B 조감경（균 P ＜0．01）；（2）B、C、D 조 MVD 급 VEGF 표체고우 A 조，PF4표체저우 A 조（균 P ＜0．01）；D 조 MVD 급 VEGF 표체저우 B 조，PF4표체고우 B 조（균 P ＜0．05）。결론：신벌타정유개선섬유화작용，기궤제가능여기조절폐조직내 VEGF 급 PF4표체、억제병이성혈관신생유관。
Objective The study was designed to observe influence of simvastatin on lung tissue angiogenesis and the gene expression of vascular endothelial growth factor(VEGF) and platelet factor 4(PF4) of rats with bleomycin (BLM)-induced pulmonary fibrosis. Methods Ninety-six healthy male SD rats were divided into four groups by random number table, including normal control group (A), bleomycin group (B), prednisone acetate treatment group (C) and simvastatin treatment group (D). Lung tissue of rats in each group was detected as specimens. HYP was detected by digestion method. Angiogenesis, VEGF and PF4 protein expression were determined by immunohistochemical method (SP). Expression of VEGF and PF4 mRNA were respectively detected by RT-PCR assay. Results (1)HYP content of group C, D was lower than the group B, which was statistical significance (P <0.01). (2)MVD and the expression of VEGF in group B, C and D was higher than that in group A. PF4 expression of group B, C and D were lower than that of group A (P < 0.01). MVD and the expression of VEGF of group D were lower than those of group B, the expression of PF4 of group D was higher than that in group B (P < 0.05). Conclusion Mechanism of simvastatin on pulmonary fibrosis may be related to regulate the expression of VEGF and PF4 in lung tissue, inhibit pathological angiogenesis.