CAJ | 학술논문

目的：研究胶质瘤干细胞中 Nrf2的表达变化及其入核表达情况，寻找胶质瘤干细胞治疗的新靶点。方法：分别用 Western 和 qPCR 从蛋白和 RNA 转录两个层次比较胶质母细胞瘤细胞系以及胶质瘤干细胞的 Nrf2表达的差异，使用免疫荧光染色初步探讨 Nrf2的活化入核情况。结果： U87和 U251裸鼠种植瘤中胶质瘤干细胞的比例分别为1．24％和1．63％。胶质瘤干细胞中的 Nrf2含量高于胶质瘤细胞系，mRNA 含量也存在相同的差异。免疫荧光显示胶质瘤干细胞中入核的 Nrf2较高。结论：Nrf2信号通路是胶质瘤干细胞功能的潜在调控因子。
목적：연구효질류간세포중 Nrf2적표체변화급기입핵표체정황，심조효질류간세포치료적신파점。방법：분별용 Western 화 qPCR 종단백화 RNA 전록량개층차비교효질모세포류세포계이급효질류간세포적 Nrf2표체적차이，사용면역형광염색초보탐토 Nrf2적활화입핵정황。결과： U87화 U251라서충식류중효질류간세포적비례분별위1．24％화1．63％。효질류간세포중적 Nrf2함량고우효질류세포계，mRNA 함량야존재상동적차이。면역형광현시효질류간세포중입핵적 Nrf2교고。결론：Nrf2신호통로시효질류간세포공능적잠재조공인자。
Objective To compare different Nrf2 expressions in glioblastoma cell lines and glioma stem cells (GSCs) from xenografts and to study the concentration of Nrf2 in nuclear. Methods GSCs were analyzed by immunofluorescence and different expressions of Nrf2 in glioblastoma cell lines and GSCs from xenografts were detected with real-time RCR and Western. Results GSCs were successfully isolated from xenografts of U251 and U87 cell lines. The percentage of tumor stem cells in total cells was 1.24%, and that was 1.63% in xenografts. Immunofluorescence indicated that Nrf2 was overexpressed in GSCs as compared with that in glioblastoma cell lines. Conclusion Nrf2 may be a potential biomarker and rational therapeutic target for GSCs.