CAJ | 학술논문

目的：探讨精氨酸多肽-环丙沙星偶联物（R11-Cipro）对细菌性膀胱炎家兔模型的治疗作用。方法：选取4月龄新西兰家兔50只，成功建立细菌性膀胱炎动物模型后，随机分为5组。 A 组为空白对照组、B 组为膀胱灌注 R11组、C 组为膀胱灌注 Cipro 组、D 组为膀胱灌注 Cipro-R11组、E 组为经耳缘静脉注射 Cipro 组。观察排尿频率、尿液细菌培养以及获取膀胱组织，HE 染色，观察其病理变化。结果：建模后膀胱黏膜上皮出现严重的炎症改变和炎症细胞浸润，R11-Cipro 组治疗后膀胱炎症改善最明显，甚至优于经静脉Cipro 组（P ＜0．05）；在排尿频率及尿细菌培养方面，R11-Cipro 组亦显著优于其余四组。结论：R11-Cipro 经膀胱灌注对家兔细菌性膀胱炎具有显著的治疗效果，R11这个高效载体能有效运载抗生素到膀胱内发挥良好的靶向杀菌活性。
목적：탐토정안산다태-배병사성우련물（R11-Cipro）대세균성방광염가토모형적치료작용。방법：선취4월령신서란가토50지，성공건립세균성방광염동물모형후，수궤분위5조。 A 조위공백대조조、B 조위방광관주 R11조、C 조위방광관주 Cipro 조、D 조위방광관주 Cipro-R11조、E 조위경이연정맥주사 Cipro 조。관찰배뇨빈솔、뇨액세균배양이급획취방광조직，HE 염색，관찰기병리변화。결과：건모후방광점막상피출현엄중적염증개변화염증세포침윤，R11-Cipro 조치료후방광염증개선최명현，심지우우경정맥Cipro 조（P ＜0．05）；재배뇨빈솔급뇨세균배양방면，R11-Cipro 조역현저우우기여사조。결론：R11-Cipro 경방광관주대가토세균성방광염구유현저적치료효과，R11저개고효재체능유효운재항생소도방광내발휘량호적파향살균활성。
Objective To investigate the therapeutic effect of ciprofloxacin (Cipro) conjugated with 11 poly-arginine peptide (R11) on rabbit model with bacterial cystitis (BC). Methods 50 New Zealand rabbits of 4-month old were chosen to establish the models and evenly divided into 5 groups randomly : Group A: normal control; Group B: intravesical instillation (II) of R11; Group C: II of Cipro; Group D: II of R11-Cipro; Group E: intravenous injection of Cipro. Several parameters were observed which included: urinary frequency, positive rate of urine culture, histopathological analysis of cystitis stained with hematoxylin and eosin. Results Severe inflammatory responses and massive infiltration of inflammatory cells were observed after the models were established. R11-Cipro group was better than intravenous injection of Cipro group in treating cystitis (P < 0.05). R11-Cipro group was better than the other four groups in urinary frequency and urine culture. Conclusions Intravesical instillation of R11-Cipro demonstrated significant therapeutic effect on bacterial cystitis. R11 , as an efficient vector, could deliver specific antibiotics to bladder mucosa precisely and function well locally.