CAJ | 학술논문

目的：观察腺病毒介导的骨形态发生蛋白-2（Ad-BMP-2）和转化生长因子-β3（Ad-TGF-β3）双基因转染骨髓间充质干细胞（BMSCs），复合脱钙骨基质（DBM）构建新型组织工程软骨修复猪关节软骨缺损的效果。方法：成年滇南小耳猪8头，16膝，制作32个全层软骨缺损模型。 A 组植入双基因转染的 BMSCs ＋DBM， B 组植入未经转染的 BMSCs ＋ DBM，C 组单纯植入 DBM，D 组为空白对照；术后2、4、8、12周取材进行形态学和组织学观察。结果：术后12周，A 组缺损区被软骨样组织修复，HE 染色显示有典型的透明软骨结构，D 组软骨缺损主要由纤维组织充填。O′driscoll 评分 A、B、C、D 组组间差异有统计学意义（P ＜0．05）。结论： Ad-BMP-2和 Ad-TGF-β3双基因转染的猪 BMSCs 复合 DBM 构建的组织工程软骨可有效修复关节软骨缺损。
목적：관찰선병독개도적골형태발생단백-2（Ad-BMP-2）화전화생장인자-β3（Ad-TGF-β3）쌍기인전염골수간충질간세포（BMSCs），복합탈개골기질（DBM）구건신형조직공정연골수복저관절연골결손적효과。방법：성년전남소이저8두，16슬，제작32개전층연골결손모형。 A 조식입쌍기인전염적 BMSCs ＋DBM， B 조식입미경전염적 BMSCs ＋ DBM，C 조단순식입 DBM，D 조위공백대조；술후2、4、8、12주취재진행형태학화조직학관찰。결과：술후12주，A 조결손구피연골양조직수복，HE 염색현시유전형적투명연골결구，D 조연골결손주요유섬유조직충전。O′driscoll 평분 A、B、C、D 조조간차이유통계학의의（P ＜0．05）。결론： Ad-BMP-2화 Ad-TGF-β3쌍기인전염적저 BMSCs 복합 DBM 구건적조직공정연골가유효수복관절연골결손。
Objective To explore the repair result of full-thickness cartilage defects in diannan small-ear pig by bone mesenchymal stem cells (BMSCs) transferred with both transforming growth factor-β3(TGF-β3) and bone morphogenetic protein-2(BMP-2) gene mediated by adenovirus vector and combined with deminerized bone matrix (DBM). Methods 32 full-thickness defects from 16 knees of 8 pigs were randomly divided into 4 groups in the experiments. In group A, the animals′ lateral femoral condyle of right knee joint was repaired with DBM and BMSC infected with both Ad-TGF-β3 and Ad-BMP-2. In group B, the medial femoral condyle of right knee joint was repaired with DBM and BMSC without infection. In group C, the lateral femoral condyle of left knee joint was repaired with DBM. And the group D is control group. Morphology and histology were observed 2, 4, 8 and 12 weeks after operation. Results 12 weeks after operation, the whole defects were repaired in group A, HE staining showed typical cartilaginous structure in the repaired area. In group D, defects were not repaired but filled with fibrous tissue. The O′driscoll scores were 15.65 ± 0.11 (group A), 11.33 ± 0.22 (group B), 6.13 ± 0.15 (group C) and 5.08 ± 0.15 (group D). There was significant difference among the groups (P < 0.05). Conclusions The new type of tissue engineering scaffold that DBM combined with BMSCs transfected with both Ad-BMP-2 and Ad-TGF-β3 could induce cartilage regeneration and repair the defects.