CAJ | 학술논문

目的：比较Lipofectamine2000、lipoplexes和apoE-lipoplexes三种脂质体对Li-7肝癌细胞的转染效率。方法构建普通阳离子脂质体 lipoplexes 和载脂蛋白 apoE 修饰脂质体apoE-lipoplexes；使用Lipofectamine2000，lipoplexes和apoE-lipoplexes三种脂质体运载以EGFP为报告基因的pGenesil-1质粒转染Li-7肝癌细胞，通过荧光显微镜观察EGFP蛋白的表达，流式细胞术检测三种脂质体的转染效率。结果 Lipofectamine2000、lipoplexes和apoE-lipoplexes三种脂质体转染效率分别为（18.63±0.57）%，（24.07±0.91）%，（28.93±0.50）%，3组间两两比较均有显著性差异（P＜0.01）。结论 Li-7肝癌细胞的转染效率与脂质体的结构有关，提示脂质体结构改造可能成为提高转染效率的新策略。
목적：비교Lipofectamine2000、lipoplexes화apoE-lipoplexes삼충지질체대Li-7간암세포적전염효솔。방법구건보통양리자지질체 lipoplexes 화재지단백 apoE 수식지질체apoE-lipoplexes；사용Lipofectamine2000，lipoplexes화apoE-lipoplexes삼충지질체운재이EGFP위보고기인적pGenesil-1질립전염Li-7간암세포，통과형광현미경관찰EGFP단백적표체，류식세포술검측삼충지질체적전염효솔。결과 Lipofectamine2000、lipoplexes화apoE-lipoplexes삼충지질체전염효솔분별위（18.63±0.57）%，（24.07±0.91）%，（28.93±0.50）%，3조간량량비교균유현저성차이（P＜0.01）。결론 Li-7간암세포적전염효솔여지질체적결구유관，제시지질체결구개조가능성위제고전염효솔적신책략。
Objective To compare the transfection efficiency of the three kinds of liposomes, Lipofectamine2000, lipoplexes and apoE-lipoplexes on Li-7 hepatoma cells. Methods To construct two kinds of gene vectors, one was normal cationic liposome (lipoplexes), another was composed of liposomes (lipoplexes) and apolipoproteinE(apoE-lipoplexes). The plasmid pGenesil-1 containing EGFP reporter gene was transfected into Li-7 cells using Lipofectamine2000, lipoplexes and apoE-lipoplexes, respectively. Then the expression of EGFP was observed by fluorescence microscopy, and the transfection efficiency of the three kinds of liposomes was measured by flow cytometry. Results Transfection efficiency of Lipofectamine2000, lipoplexes and apoE-lipoplexes was (18.63±0.57)%, (24.07±0.91)%and (28.93±0.50)%separately with the difference being significant among them (P<0.01). Conclusion Transfection efficiency of the liposome on Li-7 hepatoma cells is related to its structures, indicating that the structure transformation would be a new strategy for improving the transfection efficiency.