CAJ | 학술논문

目的：研究脐带间充质干细胞对鸡卵清蛋白诱导的哮喘小鼠模型气道炎症的抑制作用。方法将48只6~8周龄雌性 BALB/c 小鼠随机分为四组，单纯哮喘组（OVA 组），干细胞治疗组（MSC 组），地塞米松治疗组（DM 组）及生理盐水对照组（NS 组）。23 d 后，支气管肺泡灌洗液(BALF)细胞计数总细胞数、嗜酸粒细胞数；取肺组织行病理切片 HE 染色观察气道周围炎细胞浸润情况。同时免疫组织化学法观察 IL-17蛋白在各组小鼠肺组织中的表达。结果同其他各组相比，单纯哮喘组肺泡灌洗液和肺组织中有大量炎性细胞浸润，而地塞米松治疗组、干细胞治疗组气道炎细胞浸润明显减轻（P<0.05），两个治疗组之间无明显差异(P>0.10)。与此结果相一致，MSC 组、DM 组中，炎性因子 IL-17表达较 OVA 组明显减少（P<0.05），而 DM 组 IL-17表达与 MSC 组相比无明显差异(P>0.10)。结论脐带间充质干细胞能够明显抑制鸡卵清蛋白诱导的哮喘小鼠气道炎症。
목적：연구제대간충질간세포대계란청단백유도적효천소서모형기도염증적억제작용。방법장48지6~8주령자성 BALB/c 소서수궤분위사조，단순효천조（OVA 조），간세포치료조（MSC 조），지새미송치료조（DM 조）급생리염수대조조（NS 조）。23 d 후，지기관폐포관세액(BALF)세포계수총세포수、기산립세포수；취폐조직행병리절편 HE 염색관찰기도주위염세포침윤정황。동시면역조직화학법관찰 IL-17단백재각조소서폐조직중적표체。결과동기타각조상비，단순효천조폐포관세액화폐조직중유대량염성세포침윤，이지새미송치료조、간세포치료조기도염세포침윤명현감경（P<0.05），량개치료조지간무명현차이(P>0.10)。여차결과상일치，MSC 조、DM 조중，염성인자 IL-17표체교 OVA 조명현감소（P<0.05），이 DM 조 IL-17표체여 MSC 조상비무명현차이(P>0.10)。결론제대간충질간세포능구명현억제계란청단백유도적효천소서기도염증。
Objective To study the Inflammation inhibition effect of umbilical cord mesenchymal stem cells transplantation on the asthmatic mice induced by chicken ovalbumin. Methods Forty male BALB/c mice were equally randomized into four groups. The asthma group; the ucMSC intervention group; the dexamethasone treatment groups and the blank control group. Mice were sacrificed on day 23. BALF were obtained and centrifuged to pellets and super natants. The total number of cells and the eosinophils in BALF was counted. The paraffin embedded sections of lung tissue were stained with HE. The expression of IL-17 in all groups were detected by immunohistochemical method. Results Compared with the other groups, there were a great number of inflammatory cell infiltrations in the asthma group. However, there was no obvious inflammatory cells infiltration in ucMSCs control group and the dexamethasone treatment groups(P<0.05). Compared with ucMSC intervention group, the inflammatory cells infiltration decreased remarkably in dexamethasone treatment groups. Consistent with the above results, the expression of IL-17 were reduced in the ucMSC intervention group and dexamethasone treatment groups, and more obvious in dexamethasone treatment groups. Conclusion Airway inflammation of the mice induced by chicken ovalbumin were significantly reduced by exogenous ucMSC.