中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2014年
10期
83-85
,共3页
癫痫清颗粒%α-细辛醚%高效液相色谱法%含量测定
癲癇清顆粒%α-細辛醚%高效液相色譜法%含量測定
전간청과립%α-세신미%고효액상색보법%함량측정
Dianxianqing Granules%α-asarone%HPLC%content determination
目的：探讨癫痫清颗粒中α-细辛醚含量测定方法。方法采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm),以甲醇(A)-水(B)为流动相,梯度洗脱,流速为1 mL/min,紫外检测器检测波长为257 nm,柱温为25℃。结果采用70%乙醇回流提取20 min制备供试品溶液,α-细辛醚在进样量1.24×10-3～7.44×10-3μg范围内与峰面积呈良好的线性关系(r＝0.9995),精密度、稳定性、重复性均较好,平均加样回收率为98.14%,RSD＝2.5%。结论本试验为中药复方制剂中α-细辛醚的测定提供了准确、快速的分析方法。
目的：探討癲癇清顆粒中α-細辛醚含量測定方法。方法採用Kromasil C18色譜柱(250 mm×4.6 mm,5μm),以甲醇(A)-水(B)為流動相,梯度洗脫,流速為1 mL/min,紫外檢測器檢測波長為257 nm,柱溫為25℃。結果採用70%乙醇迴流提取20 min製備供試品溶液,α-細辛醚在進樣量1.24×10-3～7.44×10-3μg範圍內與峰麵積呈良好的線性關繫(r＝0.9995),精密度、穩定性、重複性均較好,平均加樣迴收率為98.14%,RSD＝2.5%。結論本試驗為中藥複方製劑中α-細辛醚的測定提供瞭準確、快速的分析方法。
목적：탐토전간청과립중α-세신미함량측정방법。방법채용Kromasil C18색보주(250 mm×4.6 mm,5μm),이갑순(A)-수(B)위류동상,제도세탈,류속위1 mL/min,자외검측기검측파장위257 nm,주온위25℃。결과채용70%을순회류제취20 min제비공시품용액,α-세신미재진양량1.24×10-3～7.44×10-3μg범위내여봉면적정량호적선성관계(r＝0.9995),정밀도、은정성、중복성균교호,평균가양회수솔위98.14%,RSD＝2.5%。결론본시험위중약복방제제중α-세신미적측정제공료준학、쾌속적분석방법。
Objective To discuss the content determination method ofα-asarone in Dianxianqing Granules. Methods Kromasil C18 column (250 mm×4.6 mm, 5 μm) was used, with methanol and water as mobile phase of gradient elution. The flow rate was 1.0 mL/min, and the detection wavelength was 257 nm. The column temperature was 25 ℃. Results The sample solution was prepared by reflux extracted with 70% ethanol for 20 min. α-asarone had a good linearity in the ranges of 1.24×10-3-7.44×10-3μg (r=0.999 5). The method was quite good in precision, stability and repeatability, and the average recovery ofα-asarone was 98.14%, with corresponding RSD of 2.5%. Conclusion The experiment provides an accurate and fast analytical method for α-asarone determination in compound preparation of traditional Chinese medicine.
