中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2014年
10期
77-79
,共3页
王玉峰%靳茂礼%李松%赵韶华%刘敏彦
王玉峰%靳茂禮%李鬆%趙韶華%劉敏彥
왕옥봉%근무례%리송%조소화%류민언
超高效液相色谱与串联四级杆飞行时间质谱仪联用技术%马钱苷%代谢产物
超高效液相色譜與串聯四級桿飛行時間質譜儀聯用技術%馬錢苷%代謝產物
초고효액상색보여천련사급간비행시간질보의련용기술%마전감%대사산물
UPLC-Q-TOF-MS/MS%loganin%metabolites
目的:采用超高效液相色谱与串联四级杆飞行时间质谱仪联用技术(UPLC-Q-TOF-MS/MS)分析鉴定大鼠灌胃马钱苷后其在尿液中的代谢产物,阐明马钱苷代谢途径。方法大鼠灌胃给予马钱苷,收集给药前后的尿样,经固相萃取进行预处理,采用超高液相色谱 C18色谱柱,流动相为乙腈-1%甲酸水,梯度洗脱,采用动态背景扣除(DBS)触发信息关联采集(IDA)进行MS/MS数据采集。结果经MetabolitePilot代谢物分析软件处理后,根据MS/MS给出质谱碎片信息对代谢产物进行结构推测,共鉴定马钱苷代谢物3种,并归纳了马钱苷在大鼠体内的代谢过程。结论该方法简单可靠,灵敏度高,可用于体内微量成分的分析鉴定。
目的:採用超高效液相色譜與串聯四級桿飛行時間質譜儀聯用技術(UPLC-Q-TOF-MS/MS)分析鑒定大鼠灌胃馬錢苷後其在尿液中的代謝產物,闡明馬錢苷代謝途徑。方法大鼠灌胃給予馬錢苷,收集給藥前後的尿樣,經固相萃取進行預處理,採用超高液相色譜 C18色譜柱,流動相為乙腈-1%甲痠水,梯度洗脫,採用動態揹景釦除(DBS)觸髮信息關聯採集(IDA)進行MS/MS數據採集。結果經MetabolitePilot代謝物分析軟件處理後,根據MS/MS給齣質譜碎片信息對代謝產物進行結構推測,共鑒定馬錢苷代謝物3種,併歸納瞭馬錢苷在大鼠體內的代謝過程。結論該方法簡單可靠,靈敏度高,可用于體內微量成分的分析鑒定。
목적:채용초고효액상색보여천련사급간비행시간질보의련용기술(UPLC-Q-TOF-MS/MS)분석감정대서관위마전감후기재뇨액중적대사산물,천명마전감대사도경。방법대서관위급여마전감,수집급약전후적뇨양,경고상췌취진행예처리,채용초고액상색보 C18색보주,류동상위을정-1%갑산수,제도세탈,채용동태배경구제(DBS)촉발신식관련채집(IDA)진행MS/MS수거채집。결과경MetabolitePilot대사물분석연건처리후,근거MS/MS급출질보쇄편신식대대사산물진행결구추측,공감정마전감대사물3충,병귀납료마전감재대서체내적대사과정。결론해방법간단가고,령민도고,가용우체내미량성분적분석감정。
Objective To identify the metabolites of loganin in rat urine through using ultra high performance liquid chromatography combined with triple TOF mass spectrometry (UPLC-Q-TOF/MS/MS) after oral administration of loganin;To summarize the metabolic pathway of loganin. Methods Rats were given a gavage with loganin. The urine samples were prepared by use of solid phase extraction (SPE). The chromatographic separation was performed on an UPLC C18 column with gradient elution program of acetonitrile and 0.1% formic acid aqueous as mobile phase. Dynamic background subtraction (DBS) technology was employed to trigger information dependent acquisition (IDA) to obtain the characteristic fragment ions of metabolites. Results MetabolitePilot software including several data processing methods were employed to speculate the structures of liganin metabolites and three metabolites were identified. Then, the metabolic pathway of loganin in rats was summarized. Conclusion The analytical method was simple, credible and highly-sensible, which is useful for screening and identifying trace constituents in vivo.