中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2014年
10期
71-73
,共3页
萆清栓%盐酸小檗碱%薄层色谱法%高效液相色谱法
萆清栓%鹽痠小檗堿%薄層色譜法%高效液相色譜法
비청전%염산소벽감%박층색보법%고효액상색보법
Biqing Suppository%berberine hydrochloride%TLC%HPLC
目的：建立萆清栓的质量标准。方法采用薄层色谱法对制剂中的萆薢、石菖蒲、丹参、黄柏进行定性鉴别。采用高效液相色谱法测定萆清栓中盐酸小檗碱的含量,色谱柱为Waters C18柱(250 mm×4.6 mm,5μm),以乙腈∶水(0.2%磷酸-0.02%三乙胺)＝25∶75为流动相,流速为1 mL/min,检测波长为265 nm。结果萆薢、石菖蒲、丹参、黄柏的薄层色谱斑点清晰,专属性强,阴性对照无干扰。盐酸小檗碱进样量在0.235～9.40μg范围内线性关系良好,回归方程 Y＝40033998.1763X－85021.2,r＝0.9997,平均回收率为99.16%,RSD＝3.45%。结论该方法简便、灵敏,重复性好,可作为萆清栓的质量控制标准。
目的：建立萆清栓的質量標準。方法採用薄層色譜法對製劑中的萆薢、石菖蒲、丹參、黃柏進行定性鑒彆。採用高效液相色譜法測定萆清栓中鹽痠小檗堿的含量,色譜柱為Waters C18柱(250 mm×4.6 mm,5μm),以乙腈∶水(0.2%燐痠-0.02%三乙胺)＝25∶75為流動相,流速為1 mL/min,檢測波長為265 nm。結果萆薢、石菖蒲、丹參、黃柏的薄層色譜斑點清晰,專屬性彊,陰性對照無榦擾。鹽痠小檗堿進樣量在0.235～9.40μg範圍內線性關繫良好,迴歸方程 Y＝40033998.1763X－85021.2,r＝0.9997,平均迴收率為99.16%,RSD＝3.45%。結論該方法簡便、靈敏,重複性好,可作為萆清栓的質量控製標準。
목적：건립비청전적질량표준。방법채용박층색보법대제제중적비해、석창포、단삼、황백진행정성감별。채용고효액상색보법측정비청전중염산소벽감적함량,색보주위Waters C18주(250 mm×4.6 mm,5μm),이을정∶수(0.2%린산-0.02%삼을알)＝25∶75위류동상,류속위1 mL/min,검측파장위265 nm。결과비해、석창포、단삼、황백적박층색보반점청석,전속성강,음성대조무간우。염산소벽감진양량재0.235～9.40μg범위내선성관계량호,회귀방정 Y＝40033998.1763X－85021.2,r＝0.9997,평균회수솔위99.16%,RSD＝3.45%。결론해방법간편、령민,중복성호,가작위비청전적질량공제표준。
Objective To establish a quality standard for Biqing Suppository. Methods Rhizoma Dioscoreae Septemlobae, Rhizoma Acori Tatarinowii, Radix Et Rhizoma Salviae Miltiorrhizae and Cortex Phellodendri Chinensis were qualitatively identified by TLC method. The content of berberine hydrochloride in Biqing Suppository was determined by HPLC method. The chromatographic column was Waters C18 (250 mm×4.6 mm, 5 μm);the mobile phase was acetonitrile∶water (0.2%phosphoric acid-0.02%three triethylamine)=25∶75;the flow rate was 1 mL/min;the detection wavelength was 265 nm. Results The spots of qualitative identification method were clear without interference. Berberine hydrochloride had good linear relationship in the range of 0.235-9.40 μg;the regression equation was Y=40 033 998.176 3X-85 021.2, r=0.999 7;the average recovery rate was 99.16%, RSD=3.45%. Conclusion The method is simple, flexible and reproducible, which can be used as the quality control standard for Biqing Suppository.
