CAJ | 학술논문

目的：采用高效液相色谱法建立同时测定葛根中葛根素、大豆苷和大豆苷元含量的方法。方法样品采用离子液体分散液相微萃取法提取。色谱条件：Phenomenex C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.2%冰醋酸溶液(45∶55),检测波长250 nm,柱温35℃,流速0.8 mL/min。结果葛根素、大豆苷和大豆苷元的质量与峰面积分别在6.24×10-6～37.44μg (r＝0.99971)、5.44×10-6～27.20μg (r＝0.99985)、5.60×10-6～28.00μg(r＝0.99994)范围内呈良好的线性关系。结论该方法快速、简便、重复性好,适于同时测定葛根中葛根素、大豆苷和大豆苷元的含量。
목적：채용고효액상색보법건립동시측정갈근중갈근소、대두감화대두감원함량적방법。방법양품채용리자액체분산액상미췌취법제취。색보조건：Phenomenex C18주(250 mm×4.6 mm,5μm),류동상위갑순-0.2%빙작산용액(45∶55),검측파장250 nm,주온35℃,류속0.8 mL/min。결과갈근소、대두감화대두감원적질량여봉면적분별재6.24×10-6～37.44μg (r＝0.99971)、5.44×10-6～27.20μg (r＝0.99985)、5.60×10-6～28.00μg(r＝0.99994)범위내정량호적선성관계。결론해방법쾌속、간편、중복성호,괄우동시측정갈근중갈근소、대두감화대두감원적함량。
Objective To develop a sensitive and specific HPLC method to simultaneously determine the contents of puerarin, daidzin and daidzein in Puerariae Lobatae Radix. Methods The three compounds were obtained by ionic liquid based on dispersive liquid phase microextraction. The determination was carried out on a Phenomenex C18 column (250 mm×4.6 mm, 5μm) with a mixture of methanol-0.2%acetic acid (volume ration 45∶55) as mobile phase at a flow rate of 0.8 mL/min. The UV detective wavelength was 250 nm, and the column temperature was set at 35 ℃. Results The linear response ranged from 6.24×10-6-37.44 μg for puerarin (r=0.999 71), 5.44×10-6-27.20μg for daidzin (r=0.999 85), and 5.60×10-6-28.00μg for daidzein (r=0.999 94), respectively. Conclusion The method is quick, simple and repeatable for simultaneous determination of the contents of puerarin, daidzin and daidzein in Puerariae Lobatae Radix.