CAJ | 학술논문

目的：探讨抵当芪桂汤防治糖尿病的作用机制。方法80只雌性SD大鼠随机抽取10只设为空白组，其余70只大鼠腹腔内一次性注射链脲佐菌素60 mg/kg造模，选取成模大鼠50只随机分为模型组、中药常量组、西药组、中药大剂量组、中药常量＋格列齐特组(中西药组)，每组10只，各给药组予相应药物灌胃。8周后，测定血清中白细胞介素(IL)-1β、IL-2的含量，并观察胰岛β细胞结构的变化。结果与模型组比较，各给药组大鼠血清 IL-1β、IL-2含量均降低，其中以中药常量组、中西药组较为明显(P＜0.05)；中药常量组、中西药组胰岛β细胞凋亡程度较轻(P＜0.05)。结论抵当芪桂汤通过抑制胰岛炎症反应，保护胰岛β细胞结构而达到防治糖尿病的作用。
목적：탐토저당기계탕방치당뇨병적작용궤제。방법80지자성SD대서수궤추취10지설위공백조，기여70지대서복강내일차성주사련뇨좌균소60 mg/kg조모，선취성모대서50지수궤분위모형조、중약상량조、서약조、중약대제량조、중약상량＋격렬제특조(중서약조)，매조10지，각급약조여상응약물관위。8주후，측정혈청중백세포개소(IL)-1β、IL-2적함량，병관찰이도β세포결구적변화。결과여모형조비교，각급약조대서혈청 IL-1β、IL-2함량균강저，기중이중약상량조、중서약조교위명현(P＜0.05)；중약상량조、중서약조이도β세포조망정도교경(P＜0.05)。결론저당기계탕통과억제이도염증반응，보호이도β세포결구이체도방치당뇨병적작용。
Objective To explore the mechanism of Didang Qigui Recipe on preventing diabetes. Methods Ten female rats were set randomly as normal group, and others 70 were injected with STZ (60 mg/kg) to establish the diabetic model. After the model was established, 50 model rats were randomly divided into five groups:the model group, Didang Qigui Recipe group, the gliclazide group, Didang Qigui Recipe high-dose group, Didang Qigui Recipe and gliclazide group, with 10 rats in each group. All groups were given a gavage with related medicine. With treatment being given respectively, eight weeks later, contents of IL-1βand IL–2 in serum were detected by ELISA and structure of pancreatic β cells was observed by immunohistochemistry and microscope. Results Compared with the model group, the contents of IL-1βand IL-2 in serum of rats decreased in treatment groups, especially in Didang Qigui Recipe group and Didang Qigui and gliclazide group (P<0.05). The degree of apoptosis in pancreaticβcells, especially in the above two groups, has been less affected than other groups (P<0.05). Conclusion Didang Qigui Recipe has preventive and therapeutic effects on diabetes by inhibition of inflammatory response and protection structure of pancreaticβcells.