CAJ | 학술논문

目的：探讨竹节参总皂苷对四氯化碳(CCl4)诱导小鼠肝损伤的保护作用。方法体外实验采用HepG2细胞株建立CCl4诱导肝细胞损伤模型。体内实验采用1%CCl4油剂处理Balb/c小鼠造成急性肝损伤模型,随机分为正常组、模型组、药物组,药物组灌胃20 mL/kg 竹节参总皂苷,正常组和模型组灌胃等量无菌生理盐水,连续8 d。MTT法观察肝细胞存活状况,HE染色观察肝组织形态,RT-PCR检测转化生长因子-β(TGF-β)、肿瘤坏死因子-α(TNF-α)和Toll样受体4(TLR4) mRNA表达。结果与正常组比较,模型组存活细胞明显减少(P＜0.01)；与模型组比较,药物组存活细胞明显增多(P＜0.01)。HE染色镜下观察,药物组小鼠肝组织损伤较模型组明显改善。RT-PCR检测结果显示,与正常组比较,模型组TGF-β、TNF-α和TLR4 mRNA表达水平明显升高；与模型组比较,药物组TGF-β、TLR4、TNF-α mRNA水平明显降低,差异有统计学意义(P＜0.01)。结论竹节参总皂苷对CCl4诱导小鼠肝损伤具有保护作用。
목적：탐토죽절삼총조감대사록화탄(CCl4)유도소서간손상적보호작용。방법체외실험채용HepG2세포주건립CCl4유도간세포손상모형。체내실험채용1%CCl4유제처리Balb/c소서조성급성간손상모형,수궤분위정상조、모형조、약물조,약물조관위20 mL/kg 죽절삼총조감,정상조화모형조관위등량무균생리염수,련속8 d。MTT법관찰간세포존활상황,HE염색관찰간조직형태,RT-PCR검측전화생장인자-β(TGF-β)、종류배사인자-α(TNF-α)화Toll양수체4(TLR4) mRNA표체。결과여정상조비교,모형조존활세포명현감소(P＜0.01)；여모형조비교,약물조존활세포명현증다(P＜0.01)。HE염색경하관찰,약물조소서간조직손상교모형조명현개선。RT-PCR검측결과현시,여정상조비교,모형조TGF-β、TNF-α화TLR4 mRNA표체수평명현승고；여모형조비교,약물조TGF-β、TLR4、TNF-α mRNA수평명현강저,차이유통계학의의(P＜0.01)。결론죽절삼총조감대CCl4유도소서간손상구유보호작용。
Objective To discuss the protection mechanism of total saponins from Panax japonicus on acute hepatic injury induced by carbon tetrachloride. Methods HepG2 cells were used to establish CCl4-induced liver cell injury model in vitro experiments. Mouse model of acute liver injury was caused by 1%CCl4 oil on Balb/c. Mice were randomly divided into normal group, model group and medicine group. Mice in the medicine group were given a gavage with 20 mL/kg total saponins from Panax japonicus, while mice in the other two groups were given a gavage with the same amount of stroke-physiological saline solution. MTT method was used to detect the activity of hepatic cells. The pathological changes of mouse liver were examined by HE staining. RT-PCR was used to detect changes in the expression of transforming growth TGF-β, TNF-α, and TLR4 mRNA. Results Compared with normal group, less hepatic cells survived in model group (P<0.01);compared with model group, more hepatic cells survived in medicine group (P<0.01). HE staining showed that damages in liver tissues of medicine group significantly improved than those in model group. RT-PCR results showed that the levels of TGF-β, TNF-α, and expression of TLR4 mRNA increased more significantly than those in model group;the expression of TGF-β, TNF-α, and TLR4 mRNA in medicine group decreased more significantly than those in model group, with statistical significance (P<0.01). Conclusion The total saponins from Panax japonicus has a protective effect on liver injury induced by carbon tetrachloride.