CAJ | 학술논문

目的：探讨PPARγC161T基因多态性及其表达与进展性脑梗死的关系。方法进展性脑梗死患者225例纳入进展组，同期住院的非进展性脑梗死患者225例为非进展组。应用多聚酶链-限制性片段长度多态性（PCR-RFLP）技术检测两组PPARγ基因C161T的基因型，酶联免疫吸附（ELISA）法检测PPARγ基因表达水平。结果进展组和非进展组患者PPARγ C161T基因多态性频率无明显差异（P>0.05）。入院时、入院后72h以及发病后4周，携带C等位基因脑梗死患者的PPARγ表达量均低于携带T基因的脑梗死患者（P<0.01），并且在携带C等位基因患者中进展组PPARγ表达量低于非进展组（P<0.01）。与入院时比较，入院后72h以及发病后4周各基因型的PPARγ表达量明显升高（P<0.01）。结论 PPARγC161T基因多态性的表达可能与进展性脑梗死的发生有关。
목적：탐토PPARγC161T기인다태성급기표체여진전성뇌경사적관계。방법진전성뇌경사환자225례납입진전조，동기주원적비진전성뇌경사환자225례위비진전조。응용다취매련-한제성편단장도다태성（PCR-RFLP）기술검측량조PPARγ기인C161T적기인형，매련면역흡부（ELISA）법검측PPARγ기인표체수평。결과진전조화비진전조환자PPARγ C161T기인다태성빈솔무명현차이（P>0.05）。입원시、입원후72h이급발병후4주，휴대C등위기인뇌경사환자적PPARγ표체량균저우휴대T기인적뇌경사환자（P<0.01），병차재휴대C등위기인환자중진전조PPARγ표체량저우비진전조（P<0.01）。여입원시비교，입원후72h이급발병후4주각기인형적PPARγ표체량명현승고（P<0.01）。결론 PPARγC161T기인다태성적표체가능여진전성뇌경사적발생유관。
Objective To investigate the relationship between C161T polymorphism on peroxisome proliferator-activated receptor gamma(PPARγ) gene and progressive ischemic stroke. Methods The C161T variants on PPARγgene of 225 patients with progressive ischemic stroke and 225 non-progressive ischemic stroke patients matched with age, gender, and past history of disease was examined by using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). The serum PPARγ was measured by ELISA. Results No statistical differences in frequencies of genotype and allele was noted between the progressive and non-progressive ischemic stroke pa-tients (P>0.05). At admission, 72h after admission and 4 weeks after onset, the C allele carriers had lower levels of PPARγ, compared with the T allele carriers (P<0.01). In C allele carriers, the PPARγ expression in progressive ischemic stroke patients was significantly lower than that in non-progressive patients(P<0.01). Compared with base-line, the PPARγ expression significantly increased in each genotype at 72h after admission and 4 weeks after onset (P<0.01). Conclusion The C161T polymorphism on PPARγ gene may be related to onset of progressive ischemic stroke.