浙江中西医结合杂志
浙江中西醫結閤雜誌
절강중서의결합잡지
ZHEJIANG JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2014年
10期
858-861
,共4页
小鼠%结肠癌人工转移模型%复方藤梨根制剂%PET/CT
小鼠%結腸癌人工轉移模型%複方籐梨根製劑%PET/CT
소서%결장암인공전이모형%복방등리근제제%PET/CT
mice%artificial blood metastasis model from colon cancer%Fufang Tengligen mixture%PET/CT
目的：观察复方藤梨根制剂对小鼠结肠癌人工转移模型的病理生理、大体解剖、分子影像及病理的影响。方法 BALB/c小鼠50只，随机分成复方藤梨根高、中、低剂量组、模型组、空白对照组5组，每组10只。除空白对照组外均尾静脉注射C26结肠腺癌细胞单细胞悬液（密度：4×106/mL）0.1mL，形成结肠癌人工血行转移模型。按照小鼠灌胃容积0.2mL/10g分别予复方藤梨根制剂2.24g/10g、1.12g/10g、0.56g/10g、生理盐水灌胃。连续灌胃21天，第1、7、14、21天观察各组小鼠一般情况，异氟烷吸入式麻醉后，进行PET扫描，通过PET图像、靶区和非靶区比值（T/N）、标准化摄取值比值（SUV）数据分析，观察各组小鼠肿瘤生长情况。第21天处死小鼠，做肺组织病理活检。结果①模型组和复方藤梨根低剂量组小鼠恶液质症状较高、中剂量组小鼠明显；②各荷瘤组T/N值和SUV值较空白对照组明显升高（P<0.01），复方藤梨根制剂各组和模型组比较，肿瘤和非肿瘤区域T/N和SUV比值较低（P<0.01），高剂量组低于中、低剂量组，差异有统计学意义（P<0.05，P<0.01）；③病理显示，各荷瘤组两肺均有转移灶，脑、肝脏、肾脏均未见明显肿瘤转移灶和癌前病变。结论经尾静脉接种C26结肠腺癌细胞可在Balb/c小鼠上形成稳定的肺转移模型，复方藤梨根制剂可以减少小鼠结肠癌人工肺转移模型的肿瘤活性，并抑制转移，有一定剂量依赖性。
目的：觀察複方籐梨根製劑對小鼠結腸癌人工轉移模型的病理生理、大體解剖、分子影像及病理的影響。方法 BALB/c小鼠50隻，隨機分成複方籐梨根高、中、低劑量組、模型組、空白對照組5組，每組10隻。除空白對照組外均尾靜脈註射C26結腸腺癌細胞單細胞懸液（密度：4×106/mL）0.1mL，形成結腸癌人工血行轉移模型。按照小鼠灌胃容積0.2mL/10g分彆予複方籐梨根製劑2.24g/10g、1.12g/10g、0.56g/10g、生理鹽水灌胃。連續灌胃21天，第1、7、14、21天觀察各組小鼠一般情況，異氟烷吸入式痳醉後，進行PET掃描，通過PET圖像、靶區和非靶區比值（T/N）、標準化攝取值比值（SUV）數據分析，觀察各組小鼠腫瘤生長情況。第21天處死小鼠，做肺組織病理活檢。結果①模型組和複方籐梨根低劑量組小鼠噁液質癥狀較高、中劑量組小鼠明顯；②各荷瘤組T/N值和SUV值較空白對照組明顯升高（P<0.01），複方籐梨根製劑各組和模型組比較，腫瘤和非腫瘤區域T/N和SUV比值較低（P<0.01），高劑量組低于中、低劑量組，差異有統計學意義（P<0.05，P<0.01）；③病理顯示，各荷瘤組兩肺均有轉移竈，腦、肝髒、腎髒均未見明顯腫瘤轉移竈和癌前病變。結論經尾靜脈接種C26結腸腺癌細胞可在Balb/c小鼠上形成穩定的肺轉移模型，複方籐梨根製劑可以減少小鼠結腸癌人工肺轉移模型的腫瘤活性，併抑製轉移，有一定劑量依賴性。
목적：관찰복방등리근제제대소서결장암인공전이모형적병리생리、대체해부、분자영상급병리적영향。방법 BALB/c소서50지，수궤분성복방등리근고、중、저제량조、모형조、공백대조조5조，매조10지。제공백대조조외균미정맥주사C26결장선암세포단세포현액（밀도：4×106/mL）0.1mL，형성결장암인공혈행전이모형。안조소서관위용적0.2mL/10g분별여복방등리근제제2.24g/10g、1.12g/10g、0.56g/10g、생리염수관위。련속관위21천，제1、7、14、21천관찰각조소서일반정황，이불완흡입식마취후，진행PET소묘，통과PET도상、파구화비파구비치（T/N）、표준화섭취치비치（SUV）수거분석，관찰각조소서종류생장정황。제21천처사소서，주폐조직병리활검。결과①모형조화복방등리근저제량조소서악액질증상교고、중제량조소서명현；②각하류조T/N치화SUV치교공백대조조명현승고（P<0.01），복방등리근제제각조화모형조비교，종류화비종류구역T/N화SUV비치교저（P<0.01），고제량조저우중、저제량조，차이유통계학의의（P<0.05，P<0.01）；③병리현시，각하류조량폐균유전이조，뇌、간장、신장균미견명현종류전이조화암전병변。결론경미정맥접충C26결장선암세포가재Balb/c소서상형성은정적폐전이모형，복방등리근제제가이감소소서결장암인공폐전이모형적종류활성，병억제전이，유일정제량의뢰성。
Objective To investigate the effect of Fufang Tengligen mixture on pathophysiology, gross anatomy, molecular imaging, and pathology of the artificial blood metastasis from colon cancer in mice. Methods Fifty BALB/c mice were randomly divided into 5 groups:Fufang Tengligen high- , middle-, and low-dose groups(2.24g/10g, 1.12 g/10g, and 0.56g/10 g,respectively), model group, and control group, 10 mice in each group. Except control group, other groups were intravenously injected with murine colon adenocarcinoma cells of 0.1 mL (4í106/mL) to make the artificial blood metastasis model.All mice in Fufang Tengligen mixture groups were given intra-gastrically with 0.2 mL/10 g medicine for 21 consecutive days. PET scanning was performed on day 1, 7, 14, and 21 after modeling. The T/N ratio, average SUV value of computer generated PET-CT images of the tumor were cal-culated for quantitive analysis. All mice were sacrificed on day 21 to perform lung biopsy. Results The symptom of cachexia was more obvious in model and Fufang Tengligen low-dose group than that in Fufang Tengligen high-and midddle-dose groups. The T/N ratio and SUV value were increased in tumor bearing groups than those in control group(P<0.01).Compared with model group, the T/N ratio and SUV value of the images of the tumor and non-tumor area were lower in Fufang Tengligen groups(P<0.01); the values were lower in Fufang Tengligen high-dose group than those in the middle-and low-dose groups(P<0.05, P<0.01). Pathological examination showed metas-tasis in both lungs of tumor bearing groups, while no metastasis in brain, liver, and kidney was seen as well as precancerous lesions. Conclusion Fufang Tengligen mixture may have anti-tumor effect on lung cancer from colon cancer in mice in a dose-dependent manner.
