CAJ | 학술논문

目的：探讨癫痫持续状态（status epilepticus，SE）大鼠模型海马区miR-181b表达的变化及意义。方法随机将30只SD（Sprague-Dawley）大鼠分为实验组和对照组，各15只，实验组建立氯化锂-匹罗卡品致SE大鼠模型，对照组以生理盐水代替氯化锂-匹罗卡品，提取两组大鼠右侧海马区脑组织总miRNA和左侧海马组织总蛋白，采用Real-Time RT-PCR检测miR-181b表达及采用Western Blot方法检测caspase-3蛋白的表达变化。结果 SE组大鼠海马区脑组织miR-181b表达较对照组有明显下降（P<0.05）， caspase-3蛋白表达明显上升（P<0.05）。结论 miR-181b可能通过调控caspase-3蛋白的表达抑制SE后海马区神经元凋亡过程。
목적：탐토전간지속상태（status epilepticus，SE）대서모형해마구miR-181b표체적변화급의의。방법수궤장30지SD（Sprague-Dawley）대서분위실험조화대조조，각15지，실험조건립록화리-필라잡품치SE대서모형，대조조이생리염수대체록화리-필라잡품，제취량조대서우측해마구뇌조직총miRNA화좌측해마조직총단백，채용Real-Time RT-PCR검측miR-181b표체급채용Western Blot방법검측caspase-3단백적표체변화。결과 SE조대서해마구뇌조직miR-181b표체교대조조유명현하강（P<0.05）， caspase-3단백표체명현상승（P<0.05）。결론 miR-181b가능통과조공caspase-3단백적표체억제SE후해마구신경원조망과정。
Objective To investigate the expression of miR-181b in the hippocampus of rat with status epilepticus(SE) model. Methods The lithium-pilocapine model of status epilepticus was established in SD rats.The expression of miR-181b and caspase-3 were examined using RT-PCR and western blot separately. Results Compared with the control group,SE group showed decreased expression of miR-181b(P<0.05)as well as increase in the expression of caspase-3(P<0.05)in the hippocampus of rat. Conclusion The miR-181b may be involved in the neuronal apoptosis process in rat hippocampus after SE by regulating the expression of caspase-3,which inhibits neuronal apoptosis.