CAJ | 학술논문

目的：探讨5-氮杂胞苷对RASSF1基因去甲基化与胃癌细胞增殖和凋亡的关系。方法将SGC7901胃癌细胞分为AZA组（5-氮杂胞苷处理）和CON组（未用5-氮杂胞苷处理），比较两组细胞RASSF1基因启动子甲基化、基因表达、细胞周期和凋亡的差异。结果 CON组SGC7901胃癌细胞RASSF1基因启动子MSP处于甲基化状态，AZA组胃癌细胞未检测到甲基化，AZA组RASSF1基因mRNA和蛋白质表达水平高于CON组细胞。AZA组SGC7901胃癌细胞G0/G1期和凋亡率高于CON组细胞（P<0.05），S期、G2/M期和PI低于CON组细胞（P<0.05）。结论5-氮杂胞苷可逆转胃癌细胞RASSF1基因启动子甲基化状态，使沉默的RASSF1基因重新获得表达而抑制胃癌细胞的增殖并促进其凋亡。
목적：탐토5-담잡포감대RASSF1기인거갑기화여위암세포증식화조망적관계。방법장SGC7901위암세포분위AZA조（5-담잡포감처리）화CON조（미용5-담잡포감처리），비교량조세포RASSF1기인계동자갑기화、기인표체、세포주기화조망적차이。결과 CON조SGC7901위암세포RASSF1기인계동자MSP처우갑기화상태，AZA조위암세포미검측도갑기화，AZA조RASSF1기인mRNA화단백질표체수평고우CON조세포。AZA조SGC7901위암세포G0/G1기화조망솔고우CON조세포（P<0.05），S기、G2/M기화PI저우CON조세포（P<0.05）。결론5-담잡포감가역전위암세포RASSF1기인계동자갑기화상태，사침묵적RASSF1기인중신획득표체이억제위암세포적증식병촉진기조망。
Objective To study the effects of 5-azacytidine’s demethylation for RASSF1 gene on gastric cancer cell’s proliferation and apoptosis. Methods SGC7901 gastric cancer cells were divided into CON group(without 5-azacytidine treatment) and AZA group(with 5-azacytidine treatment). The differences of RASSF1 gene promoter’s methylation status,mRNA and protein expressions,cell cycle and apoptosis were compared. Results The RASSF1 gene was methylated in CON group with inhibited mRNA and protein expressions while they were reversed in AZA group. The S phase、G2/M phase and PI in AZA group were lower than in CONgroup,while the G0/G1 phase and apoptosis rates were higher. Conclusion 5-azacytidine could reverse RASSF1 gene promoter’s methylation and silent gene expressions,which inhibits gastric cancer cell’s proliferation and promote apoptosis.