CAJ | 학술논문

目的：研究达玛烷苷元（ dammarane sapogenins，DS-1226）对睡眠干扰（ sleep interruption， SI）致认知障碍小鼠学习记忆能力的影响。方法将ICR小鼠随机分为对照组，模型组，DS-1226低、中、高三个剂量组。用改良滚筒法对小鼠进行睡眠干扰，同时灌胃给药15 d后，用自主活动仪检测自主活动，水迷宫、避暗实验检测学习记忆能力。结果与模型组比较，DS-1226各给药组总路程、平均速度、运动总时间均增加。模型组水迷宫定位航行D5、D6潜伏期增加；与模型组和低剂量组比较，DS-1226中剂量组D6潜伏期降低，高剂量组D1-D6潜伏期降低；与中剂量组比较，DS-1226高剂量组D1-D5潜伏期降低。模型组水迷宫空间探索目标象限穿台次数及游程比率均低于对照组；DS-1226高剂量组目标象限穿台次数、目标象限游程比率及时间比率均高于模型组，目标象限穿台次数高于中剂量组，目标象限游程比率高于低剂量组。模型组避暗学习错误次数、暗室路程、暗室时间、暗室静息时间增加；与模型组比较，DS-1226低、中剂量组错误次数、暗室路程减少，高剂量组错误次数、暗室时间、暗室路程、暗室静息时间减少，明室时间增加。结论水迷宫和避暗实验结果表明DS-1226给药对SI所致小鼠学习记忆障碍有改善作用，且有剂量相关性。
목적：연구체마완감원（ dammarane sapogenins，DS-1226）대수면간우（ sleep interruption， SI）치인지장애소서학습기억능력적영향。방법장ICR소서수궤분위대조조，모형조，DS-1226저、중、고삼개제량조。용개량곤통법대소서진행수면간우，동시관위급약15 d후，용자주활동의검측자주활동，수미궁、피암실험검측학습기억능력。결과여모형조비교，DS-1226각급약조총로정、평균속도、운동총시간균증가。모형조수미궁정위항행D5、D6잠복기증가；여모형조화저제량조비교，DS-1226중제량조D6잠복기강저，고제량조D1-D6잠복기강저；여중제량조비교，DS-1226고제량조D1-D5잠복기강저。모형조수미궁공간탐색목표상한천태차수급유정비솔균저우대조조；DS-1226고제량조목표상한천태차수、목표상한유정비솔급시간비솔균고우모형조，목표상한천태차수고우중제량조，목표상한유정비솔고우저제량조。모형조피암학습착오차수、암실로정、암실시간、암실정식시간증가；여모형조비교，DS-1226저、중제량조착오차수、암실로정감소，고제량조착오차수、암실시간、암실로정、암실정식시간감소，명실시간증가。결론수미궁화피암실험결과표명DS-1226급약대SI소치소서학습기억장애유개선작용，차유제량상관성。
Objective To study the effects of dammarane sapogenins ( DS-1226 ) on sleep interruption-induced learning and memory impairment in mice.Methods 130 SPF healthy 5 -6-week old male ICR mice were randomly divided into control, model, DS-1226 low dose, DS-1226 medium dose and DS-1226 high dose groups.The behavioral alterations in open field (OF), Morris water maze (MWM) and step-through (ST) tests were detected at 15 days after rotating drum-induced sleep interruption ( SI) .Results The total distance, movement speed, total duration of movement were increased in OF test ( P<0.05, vs.the model group) after treatment.The latency of place navigation was increased from day 5 in the MWM test after 15 d sleep interruption, and the number of crossing in the target quadrant and the percent distance in target quadrant were decreased after 15 d sleep interruption ( P <0.05, vs.the control group), while the latency of place navigation was decreased, and the percent distance in target quadrant and percent time in target quadrant after high dose DS-1226 oral administration ( P<0.05, vs.the model group) were increased.Error times, distance in dark chamber, time in dark chamber and immobility time in dark chamber were increased in training of step through test ( P<0.05, vs.the control group);while these indexes were decreased after DS-1226 oral administration ( P<0.05, vs.the model group) .But there was no significant difference in the step through testing course.Conclusions The results show that orally administrated DS-1226 can ameliorate SI-induced learning and memory impairment, and there is a significant dosage-effect relationship.