中国比较医学杂志
中國比較醫學雜誌
중국비교의학잡지
CHINESE JOURNAL OF COMPARATIVE MEDICINE
2014年
10期
39-42
,共4页
龚宝勇%吴玉娥%黄姣艳%崔玉华%张钰
龔寶勇%吳玉娥%黃姣豔%崔玉華%張鈺
공보용%오옥아%황교염%최옥화%장옥
H37 Rv%豚鼠%模型,动物%皮试
H37 Rv%豚鼠%模型,動物%皮試
H37 Rv%돈서%모형,동물%피시
H37 Rv%Guinea pig%Animal model%Skin test%Tuberculosis
目的：建立结核病生物诊断试剂模型。方法通过在豚鼠腹股沟皮下单次和多次注射不同剂量灭活H37 Rv全菌体，制备结核病生物诊断试剂模型，采用0.1 mL（5 IU）标准结核菌素（ TB-PPD）对模型效果进行评价。结果三组剂量（0.2 mg/mL，0.3 mg/mL和0.5 mg/mL）致敏豚鼠均能建立模型，其中0.2 mg/mL剂量组皮试后24 h和48 h红斑纵横径值均大于0.3 mg/mL和0.5 mg/mL剂量组，致敏效果最好；多次致敏皮试红斑纵横径值与单次致敏比较无显著差异；第1次皮试后24 h红斑纵横径值大于第2次、第3次和第4次皮试后红斑纵横径值（P≤0.05）；此外，灭活H37Rv全菌体致敏后，豚鼠体重持续增加，部分豚鼠注射部位有溃烂发生。结论应用0.2 mg/mL灭活H37 Rv全菌体单次致敏豚鼠，可以建立良好的用于结核病生物诊断试剂评价的模型。增加致敏剂量和致敏次数不能增加变态反应强度。
目的：建立結覈病生物診斷試劑模型。方法通過在豚鼠腹股溝皮下單次和多次註射不同劑量滅活H37 Rv全菌體，製備結覈病生物診斷試劑模型，採用0.1 mL（5 IU）標準結覈菌素（ TB-PPD）對模型效果進行評價。結果三組劑量（0.2 mg/mL，0.3 mg/mL和0.5 mg/mL）緻敏豚鼠均能建立模型，其中0.2 mg/mL劑量組皮試後24 h和48 h紅斑縱橫徑值均大于0.3 mg/mL和0.5 mg/mL劑量組，緻敏效果最好；多次緻敏皮試紅斑縱橫徑值與單次緻敏比較無顯著差異；第1次皮試後24 h紅斑縱橫徑值大于第2次、第3次和第4次皮試後紅斑縱橫徑值（P≤0.05）；此外，滅活H37Rv全菌體緻敏後，豚鼠體重持續增加，部分豚鼠註射部位有潰爛髮生。結論應用0.2 mg/mL滅活H37 Rv全菌體單次緻敏豚鼠，可以建立良好的用于結覈病生物診斷試劑評價的模型。增加緻敏劑量和緻敏次數不能增加變態反應彊度。
목적：건립결핵병생물진단시제모형。방법통과재돈서복고구피하단차화다차주사불동제량멸활H37 Rv전균체，제비결핵병생물진단시제모형，채용0.1 mL（5 IU）표준결핵균소（ TB-PPD）대모형효과진행평개。결과삼조제량（0.2 mg/mL，0.3 mg/mL화0.5 mg/mL）치민돈서균능건립모형，기중0.2 mg/mL제량조피시후24 h화48 h홍반종횡경치균대우0.3 mg/mL화0.5 mg/mL제량조，치민효과최호；다차치민피시홍반종횡경치여단차치민비교무현저차이；제1차피시후24 h홍반종횡경치대우제2차、제3차화제4차피시후홍반종횡경치（P≤0.05）；차외，멸활H37Rv전균체치민후，돈서체중지속증가，부분돈서주사부위유궤란발생。결론응용0.2 mg/mL멸활H37 Rv전균체단차치민돈서，가이건립량호적용우결핵병생물진단시제평개적모형。증가치민제량화치민차수불능증가변태반응강도。
Objective To establish a guinea pig model for diagnostic reagent of tuberculosis.Methods By single or multiple subcutaneous injection of heat-killed H37 Rv in different doses in the groin of guinea pigs to establish a model of positive response to 0.1 mL (5 IU) standard tuberculin ( TB-PPD) skin test.Results Three doses of heat-killed H37 Rv ( 0.2 mg/mL, 0.3 mg/mL and 0.5 mg/mL ) could be used to generate the model of biological diagnosis of tuberculosis.After 24 and 48 hours, the diameter of red spot by TB-PPD skin test was 15.4 ±2.3 mm when a dose of 0.2 mg/mL heat-killed H37 Rv was administered for immunizing and allergizing the guinea pigs.The biggest red spot was induced at doses of 0.3 mg/mL and 0.5 mg/mL.The test results showed that the immune response induced by multiple njection to immunizing and allergizing guinea pigs was not significantly different than that induced by single immunizing injection, and the first skin test was better than the second, third and fourth skin test (P≤0.05).In addition, the body weight of the guinea pigs was still increasing after infection with heat-killed H37 Rv, and ulcers occurred in the injection sites in some guinea pigs.Conclusions A single subcutaneous injection of 0.2 mg/mL heat-killed H37 Rv in guinea pigs can be used well to establish a reliable model for biological diagnostic reagent of tuberculosis.Increasing the sensitizing dose and multiple sensitization can not increase the intensity of the delayed-type hypersensitivity ( DTH) response.
