CAJ | 학술논문

四氧嘧啶诱导的实验性高血糖小鼠模型建立及其稳定性
사양밀정유도적실험성고혈당소서모형건립급기은정성
Establishment and stability of a hyperglycemic mouse model induced by alloxan

万方数据

中国比较医学杂志中國比較醫學雜誌 중국비교의학잡지
CHINESE JOURNAL OF COMPARATIVE MEDICINE
2014年 10期 32-38 ,共7页

陈琳%乐凯%茹琴%田香%熊琪%马宝苗%刘璐%吴日辉%邢俊俏%王宁%张琨%赵小伟%陈卫%何丽%欧阳康乐%司远仁%李超英

진림%악개%여금%전향%웅기%마보묘%류로%오일휘%형준초%왕저%장곤%조소위%진위%하려%구양강악%사원인%리초영

四氧嘧啶%实验性高血糖小鼠模型%剂量%溶媒%稳定性四氧嘧啶%實驗性高血糖小鼠模型%劑量%溶媒%穩定性
사양밀정%실험성고혈당소서모형%제량%용매%은정성
Alloxan%Experimental hyperglycemic mouse model%Drug dosage%Solvent%Stability

目的：探讨剂量、溶媒等主要影响因素对四氧嘧啶致实验性高血糖小鼠模型建立及稳定性的影响。方法以SPF级昆明种小鼠为实验对象，连续6周观察四氧嘧啶剂量、溶媒的不同组合对造模成功率、存活率、体重、空腹血糖值、血糖曲线下面积的稳定性及血清胰岛素水平的影响。结果160 mg/kg 体重四氧嘧啶（ pH值为4.5的柠檬酸钠缓冲液溶解），单次腹腔注射，可获得成模率70％、存活率75％、空腹血糖（15～20 mmol/L）及血糖曲线下面积（55～65 mmol/L）持续6周稳定，血清胰岛素水平（21 mIU/L）显著降低但不丧失的实验性高血糖小鼠模型。结论本实验充分考虑剂量、溶媒等主要影响因素对四氧嘧啶致实验性高血糖小鼠模型的影响，并进行6周的模型稳定性动态观察，筛选出理想的造模条件，可用于实验性糖尿病的基础研究及辅助降血糖的功能研究。
목적：탐토제량、용매등주요영향인소대사양밀정치실험성고혈당소서모형건립급은정성적영향。방법이SPF급곤명충소서위실험대상，련속6주관찰사양밀정제량、용매적불동조합대조모성공솔、존활솔、체중、공복혈당치、혈당곡선하면적적은정성급혈청이도소수평적영향。결과160 mg/kg 체중사양밀정（ pH치위4.5적저몽산납완충액용해），단차복강주사，가획득성모솔70％、존활솔75％、공복혈당（15～20 mmol/L）급혈당곡선하면적（55～65 mmol/L）지속6주은정，혈청이도소수평（21 mIU/L）현저강저단불상실적실험성고혈당소서모형。결론본실험충분고필제량、용매등주요영향인소대사양밀정치실험성고혈당소서모형적영향，병진행6주적모형은정성동태관찰，사선출이상적조모조건，가용우실험성당뇨병적기출연구급보조강혈당적공능연구。
Objective To explore the influence of drug dosage, solvent and other main influencing factors on the successful establishment of alloxan-induced hyperglycemia mouse model and the effect on the stability of this model. Methods 160 6-8-week-old Kunming mice ofSPF grade, (male:female=1:1) were used in this study.The influences of different dosages of alloxan and solvent combinations on the successful establishment rate of the model, survival rate, body weight, fasting blood glucose, blood glucose area under curve, serum insulin level and their stabilities were dynamically observed for six weeks.Results By single intraperitoneal injection of 160 mg/kg bw alloxan ( pH 4.5 citrate sodium as solvent) , we were able to obtain a stable experimental hyperglycemic mouse model with higher levels of successful establishment rate (70%), survival rate (75%), fasting blood glucose (15-20 mmol/L), glucose area under the curve (55-65 mmol/L) and a lower but not loss of serum insulin levels (21 mIU/L).Conclusions In the present study we have carefully considered the influence of main factors such as drug dosages, solvent, etc., on the alloxan-induced experimental hyperglycemic mouse model, and successfully established this model after 6-week period observation of its stability.This model may provide a useful tool in the research of experimental diabetes and hypoglycemic functional studies.