CAJ | 학술논문

目的：对一元包装过氧乙酸消毒剂的急性和亚急性毒性进行研究，为其安全使用提供依据。方法依据卫生部《消毒技术规范》（2002年版）进行试验：（1）急性毒性试验：选用健康Wistar大鼠60只，随机分组，一次性经口灌胃不同剂量消毒剂，观察大鼠的中毒症状和死亡情况，计算半数致死量（ LD50）。（2）亚急性毒性试验：选用健康Wistar大鼠40只，随机分为3个剂量组和阴性对照组，连续经口灌胃（33～342） mg/kg体重消毒剂28 d，试验结束后检测大鼠体重、脏/体比值、血液学指标及血清生化指标，并进行病理组织学检查。结果对雌性、雄性大鼠LD50分别为1470 mg/kg体重、1710 mg/kg体重；大鼠亚急性试验各剂量组体重、血液学指标、生化指标、脏/体比值，与阴性对照组比较，统计学上均差异无显著性；大体解剖观察未见异常，未见与受试物有关的病理组织学改变。结论该过氧乙酸消毒剂对大鼠急性经口毒性为低毒级，且在本试验剂量范围内，未观察到明显的亚急性经口毒性。
목적：대일원포장과양을산소독제적급성화아급성독성진행연구，위기안전사용제공의거。방법의거위생부《소독기술규범》（2002년판）진행시험：（1）급성독성시험：선용건강Wistar대서60지，수궤분조，일차성경구관위불동제량소독제，관찰대서적중독증상화사망정황，계산반수치사량（ LD50）。（2）아급성독성시험：선용건강Wistar대서40지，수궤분위3개제량조화음성대조조，련속경구관위（33～342） mg/kg체중소독제28 d，시험결속후검측대서체중、장/체비치、혈액학지표급혈청생화지표，병진행병리조직학검사。결과대자성、웅성대서LD50분별위1470 mg/kg체중、1710 mg/kg체중；대서아급성시험각제량조체중、혈액학지표、생화지표、장/체비치，여음성대조조비교，통계학상균차이무현저성；대체해부관찰미견이상，미견여수시물유관적병리조직학개변。결론해과양을산소독제대대서급성경구독성위저독급，차재본시험제량범위내，미관찰도명현적아급성경구독성。
Objective To evaluate the use safety of a unitary package peracetic acid ( PAA) disinfectant through acute and subacute toxicity experiments in rats.Methods This study was conducted according to the Technical Standard For disinfection ( Version 2002 ) .In the acute toxicity experiment, 60 healthy Wistar rats were randomly divided into different groups of PAA given by single intragastric gavage.Symptoms of poisoning of the animals were observed to calculate the half lethal dose ( LD50 ) .In the subacute toxicity experiment, 40 healthy Wistar rats were randomly divided into three dose groups and negative control group.The rats were given 33-342 mg/kg BW PAA for 28 days.At the end of test, the body weight, the organ-to-body weight ratio and the hematological and biochemical indices were determined, and pathological examination was performed.Results The acute oral LD50 of female rats was 1470 mg/kg BW, and the LD50 of male rats was 1710 mg/kg bw.The results of subacute toxicity test showed that the body weight, the organ-to-body weight ratio and the hematological and biochemical indices had no significant difference between the dose groups and control group.No obvious abnormality was observed in gross anatomy and histopathological examination.Conclusions The results of the acute toxicity test prove that PAA has a low toxicity level, and no significant subacute toxicity was observed at the exposure doses.