CAJ | 학술논문

为直接用花蕾大小或花药的形态特征来判断小孢子发育时期，对烟草吉烟9号品种小孢子发育的细胞学特征，以及小孢子不同发育时期与花冠特征、花蕾大小和花药颜色的关系进行了研究。结果表明：烟草（吉烟9号）小孢子发育经历四分体时期、单核期和双核期，各时期特征明显。吉烟9号品种的小孢子发育时期与花器官形态具有相关性。小孢子处于单核靠边期时花蕾长度1.5～2.0 cm ，此时期花药长度为0.40～0.42 cm、绿色-淡绿色，花丝长度为0.5～0.8 cm、绿色。因此，根据花器官形态特征可判断小孢子的发育时期，从而确定花药培养最佳时期所对应的选蕾标准。
위직접용화뢰대소혹화약적형태특정래판단소포자발육시기，대연초길연9호품충소포자발육적세포학특정，이급소포자불동발육시기여화관특정、화뢰대소화화약안색적관계진행료연구。결과표명：연초（길연9호）소포자발육경력사분체시기、단핵기화쌍핵기，각시기특정명현。길연9호품충적소포자발육시기여화기관형태구유상관성。소포자처우단핵고변기시화뢰장도1.5～2.0 cm ，차시기화약장도위0.40～0.42 cm、록색-담록색，화사장도위0.5～0.8 cm、록색。인차，근거화기관형태특정가판단소포자적발육시기，종이학정화약배양최가시기소대응적선뢰표준。
In order to directly judge the development stage of microspore by bud size or the morphological characteristics of anthers, the cytology of microspore at development stage and the relationships of the development period of microspore to corolla characteristics, bud size and anther color of cv. Jiyan9 were studied. The results showed that the development of microspore went through tetrad stage, uninucleate stage to binucleate stage, each stage possessed distinctive characteristics; and microspore development stage correlated to the morphology of flower organ. At the late uninucleate stage, bud was 1.5-2.0 cm in length, anther was green-pea green in color and 0.40-0.42 cm in length, and filament was green and 0.5-0.8 cm in length. Therefore, the development stage of microspore can be judged by the morphological characteristics of flower organs, and then set a standard of bud selection relative to the best stage for anther culture.