CAJ | 학술논문

采用中红外光谱技术建立了一种原位快速测定烟草木质素含量的方法，并分别测定了烟梗粉和烟叶粉的木质素含量。利用烟草木质素在1508 cm-1的吸收峰建立了烟草木质素的标准曲线。结果表明：标准曲线的拟合决定系数为0.9914；利用该方法测定的“双喜”特色优质白肋烟B1F烟梗粉和烟叶粉的木质素含量分别为2.45%和1.21%，相对标准偏差分别为3.88%和4.17%，平均加标回收率在96.96%~106.01%之间；该方法操作简便，重现性好，能快速测定烟草中的木质素含量。
채용중홍외광보기술건립료일충원위쾌속측정연초목질소함량적방법，병분별측정료연경분화연협분적목질소함량。이용연초목질소재1508 cm-1적흡수봉건립료연초목질소적표준곡선。결과표명：표준곡선적의합결정계수위0.9914；이용해방법측정적“쌍희”특색우질백륵연B1F연경분화연협분적목질소함량분별위2.45%화1.21%，상대표준편차분별위3.88%화4.17%，평균가표회수솔재96.96%~106.01%지간；해방법조작간편，중현성호，능쾌속측정연초중적목질소함량。
An in situ method for rapidly determining the lignin content in tobacco was developed with mid-infrared (MIR) spectrometry, and the lignin contents in ground tobacco stem and leaf were separately determined by this method. The calibration curve of the lignin content in tobacco was obtained on the basis of absorption peak area of lignin at 1508 cm-1 with determination coefficient of 0.9914. The results showed that the lignin contents in the ground stem and leaf of B1F burley tobacco in“Shuangxi”brand were 2.45% and 1.21% with the relative standard deviations of 3.88% and 4.17%, respectively. The average recoveries of this method ranged from 96.96% to 106.01%. With good reproducibility, this method is simple in operation and rapid for determining the lignin content in tobacco.