食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2014年
10期
3190-3196
,共7页
张小莉%魏玲%李宝明%程小艳%李萌%武会娟
張小莉%魏玲%李寶明%程小豔%李萌%武會娟
장소리%위령%리보명%정소염%리맹%무회연
肉类掺假%定量检测%溯源分析
肉類摻假%定量檢測%溯源分析
육류참가%정량검측%소원분석
meat adulteration%quantitative detection%traceable analysis
肉制品在食品消费中占很大比例,近年来,肉制品掺假问题日益严重,肉制品掺假鉴别和溯源分析成为研究热点。传统的通过感官及形态的肉类鉴别方法已经不能满足肉类掺假鉴别的需要。随着现代分析仪器和生物技术的发展,多种蛋白质分析法、DNA分析法和无损检测方法日趋成熟。基于蛋白质的检测方法,如免疫分析法和色谱分析法均可定量检测,但不适用于加工肉制品。基于样品特征光谱的无损检测方法操作简单,检测时间短,具有独特的优势。基于DNA的检测方法,特别是荧光定量PCR方法具有检测灵敏度高,结果重复性好等优势,将成为未来肉类物种鉴别的发展方向,具有很好的应用前景。
肉製品在食品消費中佔很大比例,近年來,肉製品摻假問題日益嚴重,肉製品摻假鑒彆和溯源分析成為研究熱點。傳統的通過感官及形態的肉類鑒彆方法已經不能滿足肉類摻假鑒彆的需要。隨著現代分析儀器和生物技術的髮展,多種蛋白質分析法、DNA分析法和無損檢測方法日趨成熟。基于蛋白質的檢測方法,如免疫分析法和色譜分析法均可定量檢測,但不適用于加工肉製品。基于樣品特徵光譜的無損檢測方法操作簡單,檢測時間短,具有獨特的優勢。基于DNA的檢測方法,特彆是熒光定量PCR方法具有檢測靈敏度高,結果重複性好等優勢,將成為未來肉類物種鑒彆的髮展方嚮,具有很好的應用前景。
육제품재식품소비중점흔대비례,근년래,육제품참가문제일익엄중,육제품참가감별화소원분석성위연구열점。전통적통과감관급형태적육류감별방법이경불능만족육류참가감별적수요。수착현대분석의기화생물기술적발전,다충단백질분석법、DNA분석법화무손검측방법일추성숙。기우단백질적검측방법,여면역분석법화색보분석법균가정량검측,단불괄용우가공육제품。기우양품특정광보적무손검측방법조작간단,검측시간단,구유독특적우세。기우DNA적검측방법,특별시형광정량PCR방법구유검측령민도고,결과중복성호등우세,장성위미래육류물충감별적발전방향,구유흔호적응용전경。
As a large proportion of food consumption, meat products play an important role in the day life. In recent years, meat adulteration problem is increasingly serious, and identification and traceability of meat adulteration analysis become a hot topic. Traditional meat sensory and morphological identification methods cannot meet the needs of meat authentication. With the development of modern analytical instrumentation and biotechnology, a variety of protein analysis, DNA analysis and nondestructive testing technology become more sophisticated. Protein-based detection methods, such as immunoassay and chromatography, can detect quanti-tatively, but not for processed meat. Non-destructive testing method based on the spectral characteristics of the sample is simple, short detection time, has a unique advantage. DNA-based detection methods, especially quantitative PCR detection method has high sensitivity, good reproducibility of the results and other advantages, will become the future direction of the meat species identification, and has good prospects.