CAJ | 학술논문

目的：制备原料乳中β-内酰胺酶的快速检测试剂盒,方法β-内酰胺酶分解青霉素钾的产物青霉噻唑酸具有还原性,能将二价铜还原为一价铜,2,2′-联喹啉与一价铜反应生成的是紫红色配合物,颜色深浅与β-内酰胺酶的量在一定范围内呈线性关系。结果利用可见分光光度法优化出试剂盒最佳检测条件为：0.05%2,2′-联喹啉添加量为2.4 mL,20 mmol/L的CuSO4最佳添加量0.2 mL,原料乳添加量为0.1 mL；配制β-内酰胺酶的标准品浓度梯度溶液0～200 U/mL进行显色反应后,利用分光测色仪测定颜色反应L*、a*和b*值数据,并应用色彩模块软件对其进行模拟,制作标准比色卡；原料乳样品显色15 min后与标准比色卡对比,确定牛奶中β-内酰胺酶浓度,试剂盒最低检出限为4 U/mL。结论β-内酰胺酶检测试剂盒法具有快速、操作简单、经济实用等特点,易于普及和现场测定。
목적：제비원료유중β-내선알매적쾌속검측시제합,방법β-내선알매분해청매소갑적산물청매새서산구유환원성,능장이개동환원위일개동,2,2′-련규람여일개동반응생성적시자홍색배합물,안색심천여β-내선알매적량재일정범위내정선성관계。결과이용가견분광광도법우화출시제합최가검측조건위：0.05%2,2′-련규람첨가량위2.4 mL,20 mmol/L적CuSO4최가첨가량0.2 mL,원료유첨가량위0.1 mL；배제β-내선알매적표준품농도제도용액0～200 U/mL진행현색반응후,이용분광측색의측정안색반응L*、a*화b*치수거,병응용색채모괴연건대기진행모의,제작표준비색잡；원료유양품현색15 min후여표준비색잡대비,학정우내중β-내선알매농도,시제합최저검출한위4 U/mL。결론β-내선알매검측시제합법구유쾌속、조작간단、경제실용등특점,역우보급화현장측정。
Objective To make the kit for rapid detection ofβ-lactamase in raw milk.Methods Penicil-loic acid, the decomposer of penicillin G potassium byβ-lactamase, has the reducibility and can reduce Cu2+ into Cu+, the 2, 2'-biquinolyl can coupling with Cu+to display purple, which the color depth and the amount ofβ-lactamase are in linearity within a certain range.Results Using visible spectrophotometry to optimize the best detection condition of box for: 0.05% 2, 2'-Alliance quinoline added volume for 2.4 mL, 20 mmol/L of CuSO4 for 0.2 mL, raw dairy samples added volume for 0.1 mL;β-Lactamase standard solution for gradient concentration 0~200 U/mL for colour reaction is prepared, using spectro colorimeter to determine the color reaction L*, and a* and b* value data, and applicate the color module software on its for simulation to prepare the standard color card. After 15 min for Color rendering of sample, it is compared to the standard color cards to judge the concentrations ofβ-lactamase in a sample. The detection limit of Kit was 4 U/mL. Conclusionβ-lactamase kit method is of rapid, simple, economical and practical, easy to popularize and field tests.